Significant Expansion of Fluorescent Protein Sensing Ability through the Genetic Incorporation of Superior Photo-Induced Electron-Transfer Quenchers
文献类型：期刊论文
| 作者 | Liu, Xiaohong1; Jiang, Li1; Li, Jiasong1; Wang, Li1; Yu, Yang4; Zhou, Qing1; Lv, Xiaoxuan1; Gong, Weimin1; Lu, Yi2,3; Wang, Jiangyun1 |
| 刊名 | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
 |
| 出版日期 | 2014-09-24 |
| 卷号 | 136期号:38页码:13094-13097 |
| 英文摘要 | Photo-induced electron transfer (PET) is ubiquitous for photosynthesis and fluorescent sensor design. However, genetically coded PET sensors are underdeveloped, due to the lack of methods to site-specifically install PET probes on proteins. Here we describe a family of acid and Mn(III) turn-on fluorescent protein (FP) sensors, named iLovU, based on PET and the genetic incorporation of superior PET quenchers in the fluorescent flavoprotein iLov. Using the iLovU PET sensors, we monitored the cytoplasmic acidification process, and achieved Mn(III) fluorescence sensing for the first time. The iLovU sensors should be applicable for studying pH changes in living cells, monitoring biogentic Mn(III) in the environment, and screening for efficient manganese peroxidase, which is highly desirable for lignin degradation and biomass conversion. Our work establishes a platform for many more protein PET sensors, facilitates the de novo design of metalloenzymes harboring redox active residues, and expands our ability to probe protein conformational dynamics. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Multidisciplinary |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | ESCHERICHIA-COLI ; ACID RESISTANCE ; LIVING CELLS ; TYROSINE ; CODE ; OXIDATION ; DYNAMICS ; REPORTER ; GREEN ; SITE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000342328200006 |
| 源URL | [http://124.16.173.210/handle/834782/2272]  |
| 专题 | 天津工业生物技术研究所_化学生物学研究 陆艺_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Lab RNA Biol, Beijing 100101, Peoples R China 2.Univ Illinois, Ctr Biophys & Computat Biol, Urbana, IL 61801 USA 3.Univ Illinois, Dept Chem, Urbana, IL 61801 USA 4.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Xiaohong,Jiang, Li,Li, Jiasong,et al. Significant Expansion of Fluorescent Protein Sensing Ability through the Genetic Incorporation of Superior Photo-Induced Electron-Transfer Quenchers[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2014,136(38):13094-13097. |
| APA | Liu, Xiaohong.,Jiang, Li.,Li, Jiasong.,Wang, Li.,Yu, Yang.,...&Wang, Jiangyun.(2014).Significant Expansion of Fluorescent Protein Sensing Ability through the Genetic Incorporation of Superior Photo-Induced Electron-Transfer Quenchers.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,136(38),13094-13097. |
| MLA | Liu, Xiaohong,et al."Significant Expansion of Fluorescent Protein Sensing Ability through the Genetic Incorporation of Superior Photo-Induced Electron-Transfer Quenchers".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 136.38(2014):13094-13097. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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