High level extracellular production of a recombinant alkaline catalase in E-coli BL21 under ethanol stress and its application in hydrogen peroxide removal after cotton fabrics bleaching
文献类型：期刊论文
| 作者 | Yu, Zhenxiao; Zheng, Hongchen; Zhao, Xingya; Li, Shufang; Xu, Jianyong; Song, Hui |
| 刊名 | BIORESOURCE TECHNOLOGY
 |
| 出版日期 | 2016-08-01 |
| 卷号 | 214页码:303-310 |
| 关键词 | Alkaline catalase Ethanol stress Triton X-100 Fed-batch fermentation Textile applications |
| 英文摘要 | The effects of induction parameters, osmolytes and ethanol stress on the productivity of the recombinant alkaline catalase (KatA) in Escherichia coli BL21 (pET26b-KatA) were investigated. The yield of soluble KatA was significantly enhanced by 2% ethanol stress. And a certain amount of Triton X-100 supplementation could markedly improved extracellular ratio of KatA. A total soluble catalase activity of 78,762 U/mL with the extracellular ratio of 92.5% was achieved by fed-batch fermentation in a 10 L fermentor, which was the highest yield so far. The purified KatA showed high stability at 50 degrees C and pH 6-10. Application of KatA for elimination of H2O2 after cotton fabrics bleaching led to less consumption of water, steam and electric power by 25%, 12% and 16.7% respectively without productivity and quality losing of cotton fabrics. Thus, the recombinant KatA is a promising candidate for industrial production and applications. (C) 2016 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 研究领域[WOS] | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 关键词[WOS] | BACILLUS-SUBTILIS WSHDZ-01 ; PURIFICATION ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000377366900040 |
| 源URL | [http://124.16.173.210/handle/834782/2901]  |
| 专题 | 天津工业生物技术研究所_基因工程与微生物应用技术研究组 宋恢_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Ind Enzymes Natl Engn Lab, Tianjin 300308, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Key Lab Ind Biol Syst & Bioproc Engn, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Zhenxiao,Zheng, Hongchen,Zhao, Xingya,et al. High level extracellular production of a recombinant alkaline catalase in E-coli BL21 under ethanol stress and its application in hydrogen peroxide removal after cotton fabrics bleaching[J]. BIORESOURCE TECHNOLOGY,2016,214:303-310. |
| APA | Yu, Zhenxiao,Zheng, Hongchen,Zhao, Xingya,Li, Shufang,Xu, Jianyong,&Song, Hui.(2016).High level extracellular production of a recombinant alkaline catalase in E-coli BL21 under ethanol stress and its application in hydrogen peroxide removal after cotton fabrics bleaching.BIORESOURCE TECHNOLOGY,214,303-310. |
| MLA | Yu, Zhenxiao,et al."High level extracellular production of a recombinant alkaline catalase in E-coli BL21 under ethanol stress and its application in hydrogen peroxide removal after cotton fabrics bleaching".BIORESOURCE TECHNOLOGY 214(2016):303-310. |
入库方式： OAI收割
来源：天津工业生物技术研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。