In vitro functional characterization of the Na+/H+ antiporters in Corynebacterium glutamicum
文献类型：期刊论文
| 作者 | Xu, Ning1; Wang, Lei2; Cheng, Haijiao1,3; Liu, Qingdai3; Liu, Jun1; Ma, Yanhe1 |
| 刊名 | FEMS MICROBIOLOGY LETTERS
 |
| 出版日期 | 2016-02-01 |
| 卷号 | 363期号:3 |
| 关键词 | Corynebactrerium glutamicum Na+/H+ antiporters function |
| 英文摘要 | Corynebacterium glutamicum, typically used as industrial workhorse for amino acid production, is a moderately salt-alkali-tolerant microorganism with optimal growth at pH 7-9. However, little is known about the mechanisms of salt-alkali tolerance in C. glutamicum. Here, the catalytic capacity of three putative Na+/H+ antiporters from C. glutamicum (Idesignated as Cg-Mrp1, Cg-Mrp2 and Cg-NhaP) were characterized in an antiporter-deficient Escherichia coli KNabc strain. Only Cg-Mrp1 was able to effectively complement the Na+-sensitive of E. coli KNabc. Cg-Mrp1 exhibited obvious Na+(Li+)/H+ antiport activities with low apparent Km values of 1.08 mM and 1.41 mM for Na+ and Li+, respectively. The Na+/H+ antiport activity of Cg-Mrp1 was optimal in the alkaline pH range. All three antiporters showed detectable K+/H+ antiport activitiy. Cg-NhaP also exhibited Na+(Li+,Rb+)/H+ antiport activities but at lower levels of activity. Interestingly, overexpression of Cg-Mrp2 exhibited clear Na+(K+)/H+ antiport activities. These results suggest that C. glutamicum Na+(K+)/H+ antiporters may have overlapping roles in coping with salt-alkali and perhaps high-osmolarity stress. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Microbiology |
| 研究领域[WOS] | Microbiology |
| 关键词[WOS] | ESCHERICHIA-COLI ; BACILLUS-SUBTILIS ; CATION/PROTON ANTIPORTERS ; ALKALIMONAS-AMYLOLYTICA ; PSEUDOMONAS-AERUGINOSA ; CATION/H+ ANTIPORTERS ; GENOME SEQUENCE ; PH HOMEOSTASIS ; ALKALINE PH ; GENE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000377305000003 |
| 源URL | [http://124.16.173.210/handle/834782/2903]  |
| 专题 | 天津工业生物技术研究所_极端微生物生理学和代谢工程课题组 刘君_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 2.China Agr Univ, Coll Biol Sci, Dept Microbiol & Immunol, Beijing 10094, Peoples R China 3.Tianjin Univ Sci & Technol, Sch Food Engn & Biotechnol, Tianjin 300457, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Ning,Wang, Lei,Cheng, Haijiao,et al. In vitro functional characterization of the Na+/H+ antiporters in Corynebacterium glutamicum[J]. FEMS MICROBIOLOGY LETTERS,2016,363(3). |
| APA | Xu, Ning,Wang, Lei,Cheng, Haijiao,Liu, Qingdai,Liu, Jun,&Ma, Yanhe.(2016).In vitro functional characterization of the Na+/H+ antiporters in Corynebacterium glutamicum.FEMS MICROBIOLOGY LETTERS,363(3). |
| MLA | Xu, Ning,et al."In vitro functional characterization of the Na+/H+ antiporters in Corynebacterium glutamicum".FEMS MICROBIOLOGY LETTERS 363.3(2016). |
入库方式： OAI收割
来源：天津工业生物技术研究所
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