Improving the catalytic activity of isopentenyl phosphate kinase through protein coevolution analysis
文献类型：期刊论文
| 作者 | Liu, Ying1,2; Yan, Zhihui2; Lu, Xiaoyun2; Xiao, Dongguang1; Jiang, Huifeng2 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2016-04-07 |
| 卷号 | 6 |
| 英文摘要 | Protein rational design has become more and more popular for protein engineering with the advantage of biological big-data. In this study, we described a method of rational design that is able to identify desired mutants by analyzing the coevolution of protein sequence. We employed this approach to evolve an archaeal isopentenyl phosphate kinase that can convert dimethylallyl alcohol (DMA) into precursor of isoprenoids. By designing 9 point mutations, we improved the catalytic activities of IPK about 8-fold in vitro. After introducing the optimal mutant of IPK into engineered E. coli strain for beta-carotenoids production, we found that beta-carotenoids production exhibited 97% increase over the starting strain. The process of enzyme optimization presented here could be used to improve the catalytic activities of other enzymes. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | MEVALONATE-INDEPENDENT PATHWAY ; MULTIPLE SEQUENCE ALIGNMENT ; ESCHERICHIA-COLI ; ISOPRENOID BIOSYNTHESIS ; BETA-CAROTENE ; EVOLUTION ; RESIDUES ; DIPHOSPHATE ; ARCHAEA ; DESIGN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000373590200001 |
| 源URL | [http://124.16.173.210/handle/834782/2240]  |
| 专题 | 天津工业生物技术研究所_酵母基因组工程课题组 江会锋_期刊论文 |
| 作者单位 | 1.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300308, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, Tianjin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Ying,Yan, Zhihui,Lu, Xiaoyun,et al. Improving the catalytic activity of isopentenyl phosphate kinase through protein coevolution analysis[J]. SCIENTIFIC REPORTS,2016,6. |
| APA | Liu, Ying,Yan, Zhihui,Lu, Xiaoyun,Xiao, Dongguang,&Jiang, Huifeng.(2016).Improving the catalytic activity of isopentenyl phosphate kinase through protein coevolution analysis.SCIENTIFIC REPORTS,6. |
| MLA | Liu, Ying,et al."Improving the catalytic activity of isopentenyl phosphate kinase through protein coevolution analysis".SCIENTIFIC REPORTS 6(2016). |
入库方式： OAI收割
来源：天津工业生物技术研究所
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