Structure and Function of a "Head-to-Middle" Prenyltransferase: Lavandulyl Diphosphate Synthase
文献类型：期刊论文
| 作者 | Liu, Meixia1; Chen, Chun-Chi2; Chen, Lu5; Xiao, Xiansha2; Zheng, Yingying2; Huang, Jian-Wen4; Liu, Weidong2; Ko, Tzu-Ping3; Cheng, Ya-Shan2; Feng, Xinxin6 |
| 刊名 | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
 |
| 出版日期 | 2016-04-04 |
| 卷号 | 55期号:15页码:4721-4724 |
| 关键词 | biosynthesis fragrance monoterpenes natural products structure elucidation |
| 英文摘要 | We report the first X-ray structure of the unique head-to-middle monoterpene synthase, lavandulyl diphosphate synthase (LPPS). LPPS catalyzes the condensation of two molecules of dimethylallyl diphosphate (DMAPP) to form lavandulyl diphosphate, a precursor to the fragrance lavandulol. The structure is similar to that of the bacterial cis-prenyl synthase, undecaprenyl diphosphate synthase (UPPS), and contains an allylic site (S1) in which DMAPP ionizes and a second site (S2) which houses the DMAPP nucleophile. Both S-thiolo-dimethylallyl diphosphate and S-thiolo-isopentenyl diphosphate bind intact to S2, but are cleaved to (thio)diphosphate, in S1. His78 (Asn in UPPS) is essential for catalysis and is proposed to facilitate diphosphate release in S1, while the P1 phosphate in S2 abstracts a proton from the lavandulyl carbocation to form the LPP product. The results are of interest since they provide the first structure and structure-based mechanism of this unusual prenyl synthase. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Multidisciplinary |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | TERPENE BIOSYNTHESIS ; MECHANISM ; SITES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000373615900016 |
| 源URL | [http://124.16.173.210/handle/834782/2242]  |
| 专题 | 天津工业生物技术研究所_结构生物学与蛋白酶学实验室 郭瑞庭_期刊论文 |
| 作者单位 | 1.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Ind Enzymes Natl Engn Lab, Tianjin 300308, Peoples R China 3.Acad Sinica, Inst Biol Chem, Taipei 11529, Taiwan 4.AsiaPac Biotechnol Co Ltd, Dongguan 523808, Peoples R China 5.Univ Illinois, Dept Biochem, Urbana, IL 61801 USA 6.Univ Illinois, Dept Chem, Urbana, IL 61801 USA |
| 推荐引用方式 GB/T 7714 | Liu, Meixia,Chen, Chun-Chi,Chen, Lu,et al. Structure and Function of a "Head-to-Middle" Prenyltransferase: Lavandulyl Diphosphate Synthase[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2016,55(15):4721-4724. |
| APA | Liu, Meixia.,Chen, Chun-Chi.,Chen, Lu.,Xiao, Xiansha.,Zheng, Yingying.,...&Ma, Yanhe.(2016).Structure and Function of a "Head-to-Middle" Prenyltransferase: Lavandulyl Diphosphate Synthase.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,55(15),4721-4724. |
| MLA | Liu, Meixia,et al."Structure and Function of a "Head-to-Middle" Prenyltransferase: Lavandulyl Diphosphate Synthase".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 55.15(2016):4721-4724. |
入库方式： OAI收割
来源：天津工业生物技术研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。