Purification, characterization and decolorization of bilirubin oxidase from Myrothecium verrucaria 3.2190
文献类型：期刊论文
| 作者 | Han, Xu1,2; Zhao, Min1; Lu, Lei1; Liu, Yanhong3 |
| 刊名 | FUNGAL BIOLOGY
 |
| 出版日期 | 2012-08-01 |
| 卷号 | 116期号:8页码:863-871 |
| 关键词 | Bilirubin oxidase Dye decolorization Myrothecium verrucaria Purification and characterization |
| 英文摘要 | Myrothecium verrucaria 3.2190 is a nonligninolytic fungus that produces bilirubin oxidase. Both M. verrucaria and the extracellular bilirubin oxidase were tested for their ability to decolorize indigo carmine. The biosorption and biodegradation of the dye were detected during the process of decolorization; more than 98% decolorization efficiency was achieved after 7 days at 26 degrees C. Additionally, the crude bilirubin oxidase can efficiently decolorize indigo carmine at 30 degrees C similar to 50 degrees C, pH 5.5 similar to 9.5 with dye concentrations of 50 mg l(-1)similar to 200 mg l(-1) Bilirubin oxidase was purified and visualized as a single band on native polyacrylamide gel electrophoresis (PAGE). Several enzymatic properties of the purified enzyme were investigated. Moreover, the identity of the purified bilirubin oxidase (BOD) was confirmed by matrix assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF-MS). These results demonstrate that the purified bilirubin oxidase in M. verrucaria strain has potential application in dye effluent decolorization. (C) 2012 The British Mycological Society. Published by Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Mycology |
| 研究领域[WOS] | Mycology |
| 关键词[WOS] | AZO DYES ; PHANEROCHAETE-CHRYSOSPORIUM ; TRAMETES-VERSICOLOR ; LACCASE ACTIVITY ; WASTE MATERIALS ; HAZARDOUS DYE ; BIODEGRADATION ; DEGRADATION ; ADSORPTION ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000308732100002 |
| 源URL | [http://124.16.173.210/handle/834782/2265]  |
| 专题 | 天津工业生物技术研究所_结构生物学与蛋白酶学实验室 郭瑞庭_期刊论文 |
| 作者单位 | 1.NE Forestry Univ, Coll Life Sci, Harbin 150040, Heilongjiang, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Airport Econ Area, Tianjin 300308, Peoples R China 3.USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA |
| 推荐引用方式 GB/T 7714 | Han, Xu,Zhao, Min,Lu, Lei,et al. Purification, characterization and decolorization of bilirubin oxidase from Myrothecium verrucaria 3.2190[J]. FUNGAL BIOLOGY,2012,116(8):863-871. |
| APA | Han, Xu,Zhao, Min,Lu, Lei,&Liu, Yanhong.(2012).Purification, characterization and decolorization of bilirubin oxidase from Myrothecium verrucaria 3.2190.FUNGAL BIOLOGY,116(8),863-871. |
| MLA | Han, Xu,et al."Purification, characterization and decolorization of bilirubin oxidase from Myrothecium verrucaria 3.2190".FUNGAL BIOLOGY 116.8(2012):863-871. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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