Enhanced alpha-Zearalenol Hydrolyzing Activity of a Mycoestrogen-Detoxifying Lactonase by Structure-Based Engineering
文献类型：期刊论文
| 作者 | Xu, Zhongxia1,2; Liu, Weidong1; Chen, Chun-Chi1; Li, Qian1; Huang, Jian-Wen3; Ko, Tzu-Ping4; Liu, Guizhi1; Liu, Wenting1; Peng, Wei1; Cheng, Ya-Shan1 |
| 刊名 | ACS CATALYSIS
 |
| 出版日期 | 2016-11-01 |
| 卷号 | 6期号:11页码:7657-7663 |
| 关键词 | mycoestrogen-detoxifying lactonase zearalenone alpha-zearalenol complex structure rational design substrate specificity |
| 英文摘要 | The enzyme ZHD101 from Clonostachys rosea hydrolyzes and deactivates the mycotoxin zearalenone (ZEN) and its zearalenol (ZOL) derivatives. ZHD101 prefers ZEN to ZOL as its substrate, but ZOL, especially the alpha-form, shows higher estrogenic toxicity than ZEN. To enhance alpha-ZOL selectivity, we solved the complex structures of ZHD101 with both ZOLs and modified several lactone-surrounding residues. Among the mutants, V153H maintained activity for ZEN but showed a 3.7-fold increase in specific activity against alpha-ZOL, with an similar to 2.7-fold reduction in substrate affinity but a 5.2-fold higher turnover rate. We then determined two V153H/ZOL complex structures. Here, the alpha-ZOL lactone ring is hydrogen bonded to the H153 side chain, yielding a larger space for H242 to reconstitute the catalytic triad. In conclusion, structure-based engineering was successfully employed to ZHD101 activity toward the more toxic alpha-ZOL, with great potential in further industrial applications. improve the |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Physical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | SACCHAROMYCES-CEREVISIAE ; MYCOTOXIN ZEARALENONE ; DETOXIFICATION GENE ; AB-INITIO ; FUSARIUM ; DECONTAMINATION ; CONTAMINATION ; DERIVATIVES ; EXPRESSION ; PK(A) |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000387306100042 |
| 源URL | [http://124.16.173.210/handle/834782/2292]  |
| 专题 | 天津工业生物技术研究所_结构生物学与蛋白酶学实验室 郭瑞庭_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Ind Enzymes Natl Engn Lab, Tianjin 300308, Peoples R China 2.Jiangnan Univ, Sch Biotechnol, Wuxi 214122, Peoples R China 3.AsiaPac Biotechnol Co Ltd, Dongguan 523808, Peoples R China 4.Acad Sinica, Inst Biol Chem, Taipei 11529, Taiwan 5.Jiangnan Univ, Sch Pharmaceut Sci, Wuxi 214122, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Zhongxia,Liu, Weidong,Chen, Chun-Chi,et al. Enhanced alpha-Zearalenol Hydrolyzing Activity of a Mycoestrogen-Detoxifying Lactonase by Structure-Based Engineering[J]. ACS CATALYSIS,2016,6(11):7657-7663. |
| APA | Xu, Zhongxia.,Liu, Weidong.,Chen, Chun-Chi.,Li, Qian.,Huang, Jian-Wen.,...&Guo, Rey-Ting.(2016).Enhanced alpha-Zearalenol Hydrolyzing Activity of a Mycoestrogen-Detoxifying Lactonase by Structure-Based Engineering.ACS CATALYSIS,6(11),7657-7663. |
| MLA | Xu, Zhongxia,et al."Enhanced alpha-Zearalenol Hydrolyzing Activity of a Mycoestrogen-Detoxifying Lactonase by Structure-Based Engineering".ACS CATALYSIS 6.11(2016):7657-7663. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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