Crystallization and preliminary X-ray diffraction analysis of YisP protein from Bacillus subtilis subsp subtilis strain 168
文献类型：期刊论文
| 作者 | Hu, Yumei1,2; Jia, Shiru1; Ren, Feifei2; Huang, Chun-Hsiang2; Ko, Tzu-Ping3; Mitchell, Douglas A.4; Guo, Rey-Ting2; Zheng, Yingying2 |
| 刊名 | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
 |
| 出版日期 | 2013 |
| 卷号 | 69页码:77-79 |
| 英文摘要 | YisP is an enzyme involved in the pathway of biofilm formation in bacteria and is predicted to possess squalene synthase activity. A BlastP search using the YisP protein sequence from Bacillus subtilis subsp. subtilis strain 168 shows that it shares 23% identity with the dehydrosqualene synthase from Staphylococcus aureus. The YisP from B. subtilis 168 was expressed in Escherichia coli and the recombinant protein was purified and crystallized. The crystals, which belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 43.966, b = 77.576, c = 91.378 angstrom, were obtained by the sitting-drop vapour-diffusion method and diffracted to 1.92 angstrom resolution. Structure determination using MAD and MIR methods is in progress. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Crystallography |
| 关键词[WOS] | HUMAN SQUALENE SYNTHASE ; CHOLESTEROL-BIOSYNTHESIS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000313055000019 |
| 源URL | [http://124.16.173.210/handle/834782/2880]  |
| 专题 | 天津工业生物技术研究所_结构生物学与蛋白酶学实验室 郭瑞庭_期刊论文 |
| 作者单位 | 1.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin Key Lab Ind Microbiol, Tianjin 300457, Peoples R China 2.Chinese Acad Sci, Ind Enzymes Natl Engn Lab, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 3.Acad Sinica, Inst Biol Chem, Taipei 11529, Taiwan 4.Univ Illinois, Dept Microbiol, Urbana, IL 62801 USA |
| 推荐引用方式 GB/T 7714 | Hu, Yumei,Jia, Shiru,Ren, Feifei,et al. Crystallization and preliminary X-ray diffraction analysis of YisP protein from Bacillus subtilis subsp subtilis strain 168[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,2013,69:77-79. |
| APA | Hu, Yumei.,Jia, Shiru.,Ren, Feifei.,Huang, Chun-Hsiang.,Ko, Tzu-Ping.,...&Zheng, Yingying.(2013).Crystallization and preliminary X-ray diffraction analysis of YisP protein from Bacillus subtilis subsp subtilis strain 168.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,69,77-79. |
| MLA | Hu, Yumei,et al."Crystallization and preliminary X-ray diffraction analysis of YisP protein from Bacillus subtilis subsp subtilis strain 168".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS 69(2013):77-79. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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