Functional and structural analysis of Pichia pastoris-expressed Aspergillus niger 1,4-beta-endoglucanase
文献类型：期刊论文
| 作者 | Yan, Junjie1; Liu, Weidong2; Li, Yujie2; Lai, Hui-Lin3,4; Zheng, Yingying2; Huang, Jian-Wen3,4; Chen, Chun-Chi2; Chen, Yun5; Jin, Jian5; Li, Huazhong1 |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2016-06-17 |
| 卷号 | 475期号:1页码:8-12 |
| 关键词 | 1,4-beta-Endoglucanase Aspergillus niger Pichia pastoris Crystal structure |
| 英文摘要 | Eukaryotic 1,4-beta-endoglucanases (EC 3.2.1.4) have shown great potentials in many commercial applications because they effectively catalyze hydrolysis of cellulose, the main component of the plant cell wall. Here we expressed a glycoside hydrolase family (GH) 5 1,4-beta-endoglucanase from Aspergillus niger (AnCel5A) in Pichia pastoris, which exhibits outstanding pH and heat stability. In order to further investigate the molecular mechanism of AnCel5A, apo-form and cellotetraose (CH) complex enzyme crystal structures were solved to high resolution. AnCel5A folds into a typical (beta/alpha)(8)-TIM barrel architecture, resembling other GH5 members. In the substrate binding cavity, CTT is found to bind to -4--1 subsites, and several polyethylene glycol molecules are found in positive subsites. In addition, several unique N-glycosylation motifs that may contribute to protein higher stability were observed from crystal structures. These results are of great importance for understanding the molecular mechanism of AnCel5A, and also provide guidance for further applications of the enzyme. (C) 2016 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | THERMOASCUS-AURANTIACUS ; ENDOGLUCANASE ; PURIFICATION ; ACCURACY ; ANGSTROM ; ENZYMES ; CLONING ; CEL5A ; YEAST ; MODE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000377557800002 |
| 源URL | [http://124.16.173.210/handle/834782/2904]  |
| 专题 | 天津工业生物技术研究所_结构生物学与蛋白酶学实验室 郭瑞庭_期刊论文 |
| 作者单位 | 1.Jiangnan Univ, Sch Biotechnol, Wuxi 214122, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 3.Genozyme Biotechnol Inc, Taipei 106, Taiwan 4.AsiaPac Biotechnol Co Ltd, Dongguan 523808, Peoples R China 5.Jiangnan Univ, Sch Pharmaceut Sci, Wuxi 214122, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yan, Junjie,Liu, Weidong,Li, Yujie,et al. Functional and structural analysis of Pichia pastoris-expressed Aspergillus niger 1,4-beta-endoglucanase[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2016,475(1):8-12. |
| APA | Yan, Junjie.,Liu, Weidong.,Li, Yujie.,Lai, Hui-Lin.,Zheng, Yingying.,...&Guo, Rey-Ting.(2016).Functional and structural analysis of Pichia pastoris-expressed Aspergillus niger 1,4-beta-endoglucanase.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,475(1),8-12. |
| MLA | Yan, Junjie,et al."Functional and structural analysis of Pichia pastoris-expressed Aspergillus niger 1,4-beta-endoglucanase".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 475.1(2016):8-12. |
入库方式： OAI收割
来源：天津工业生物技术研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。