Crystal structure and genetic modifications of FI-CMCase from Aspergillus aculeatus F-50
文献类型：期刊论文
| 作者 | Huang, Jian-Wen1; Liu, Weidong2; Lai, Hui-Lin1; Cheng, Ya-Shan1; Zheng, Yingying2; Li, Qian2; Sun, Hong2; Kuo, Chih-Jung3; Guo, Rey-Ting2; Chen, Chun-Chi2 |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2016-09-16 |
| 卷号 | 478期号:2页码:565-572 |
| 关键词 | Endoglucanase Aspergillus aculeatus Protein structure X-ray crystallography |
| 英文摘要 | Cellulose is the major component of the plant cell wall and the most abundant renewable biomass on earth, and its decomposition has proven to be very useful in many commercial applications. Endo-1,4-beta-D-glucanase (EC 3.2.1.4; endoglucanase), which catalyzes the random hydrolysis of 1,4-beta-glycosidic bonds of the cellulose main chain to cleave cellulose into smaller fragments, is the key cellulolytic enzyme. An endoglucanase isolated from Aspergillus aculeatus F-50 (FI-CMCase), which is classified into the glycoside hydrolase (GH) family 12, was demonstrated to be effectively expressed in the industrial strain Pichia pastoris. Here, the crystal structure and complex structures of P. pastoris-expressed FI-CMCase were solved to high resolution. The overall structure is analyzed and compared to other GH12 members. In addition, the substrate-surrounding residues were engineered to search for variants with improved enzymatic activity. Among 14 mutants constructed, one with two-fold increase in protein expression was identified, which possesses a potential to be further developed as a commercial enzyme product. (C) 2016 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | X-RAY-DIFFRACTION ; PICHIA-PASTORIS ; SACCHAROMYCES-CEREVISIAE ; RECOMBINANT PROTEINS ; EXPRESSION ; CRYSTALLIZATION ; PURIFICATION ; CELLULASES ; SEQUENCE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000383528600010 |
| 源URL | [http://124.16.173.210/handle/834782/2926]  |
| 专题 | 天津工业生物技术研究所_结构生物学与蛋白酶学实验室 郭瑞庭_期刊论文 |
| 作者单位 | 1.AsiaPac Biotechnol Co Ltd, Dongguan 523808, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Ind Enzymes Natl Engn Lab, Tianjin 300308, Peoples R China 3.Natl Chung Hsing Univ, Dept Vet Med, Taichung 402, Taiwan |
| 推荐引用方式 GB/T 7714 | Huang, Jian-Wen,Liu, Weidong,Lai, Hui-Lin,et al. Crystal structure and genetic modifications of FI-CMCase from Aspergillus aculeatus F-50[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2016,478(2):565-572. |
| APA | Huang, Jian-Wen.,Liu, Weidong.,Lai, Hui-Lin.,Cheng, Ya-Shan.,Zheng, Yingying.,...&Chen, Chun-Chi.(2016).Crystal structure and genetic modifications of FI-CMCase from Aspergillus aculeatus F-50.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,478(2),565-572. |
| MLA | Huang, Jian-Wen,et al."Crystal structure and genetic modifications of FI-CMCase from Aspergillus aculeatus F-50".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 478.2(2016):565-572. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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