Integrating MS1 and MS2 Scans in High-Resolution Parallel Reaction Monitoring Assays for Targeted Metabolite Quantification and Dynamic C-13-Labeling Metabolism Analysis
文献类型：期刊论文
| 作者 | Li, Zhucui1,2; Li, Yujing3; Chen, Wujiu1; Cao, Qichen1; Guo, Yufeng1; Wan, Ni4; Jiang, Xiaolong1; Tang, Yinjie J.4; Wang, Qinhong1; Shui, Wenqing2 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2017-01-03 |
| 卷号 | 89期号:1页码:877-885 |
| 英文摘要 | Quantification of 'targeted metabolites, especially trace- metabolites and structural isomers, in complex biological anaterials is an ongoing challenge for metabolomics. Initially developed for proteomic analysis, the parallel reaction monitoring (PRM) technique exploiting high-resolution MS2 fragment ion data..has shown high". promise for targeted metabolite quantification. Notably, MS1 ion interisity data acquired independently as part of each PRM scan cycle are often-underutilized in the PRM assay. In this study, we developed ari MS1/MS2-combined PRM workflow, for, quantification of central carbon- metabolism intermediates,, amino acids and shikimate pathway-related metabolites on an orthogonal QqTOF system. Concentration curve assessment revealed that exploiting both MS1 and MS2 scans in PRM analysis afforded higher sensitivity, :wider dynamic range and better reproducibility than relying on either scan mode for quantification. Furthermore, Skyline was incorporated into out workflow to process the MSS/MS2 ion intensity data, and eliminate noisy signals and transitions with interferences. This iritegrated MS1/MS2 PRM approach was applied to targeted metabolite quantification in engineered E. coil strains for understanding of metabolic pathway modulation. In addition, this new approach, when first implemented in a dynamic C-13-labeling experiment, showed its unique advantage in capturing arid correcting isotopomer labeling curves to facilitate nonstationary C-13-labeling metabolism analysis. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | QUANTITATIVE BIOMARKER ANALYSIS ; SPECTROMETRY-BASED METABOLOMICS ; MASS-SPECTROMETER ; ESCHERICHIA-COLI ; FLUX ANALYSIS ; LC-MS ; SCALE ; IDENTIFICATION ; CHROMATOGRAPHY ; ACQUISITION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000391346600078 |
| 源URL | [http://124.16.173.210/handle/834782/2277]  |
| 专题 | 天津工业生物技术研究所_进化代谢工程和分子进化研究组 王钦宏_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 2.ShanghaiTech Univ, iHuman Inst, Shanghai 201210, Peoples R China 3.Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China 4.Washington Univ, Dept Energy Environm & Chem Engn, St Louis, MO 63130 USA |
| 推荐引用方式 GB/T 7714 | Li, Zhucui,Li, Yujing,Chen, Wujiu,et al. Integrating MS1 and MS2 Scans in High-Resolution Parallel Reaction Monitoring Assays for Targeted Metabolite Quantification and Dynamic C-13-Labeling Metabolism Analysis[J]. ANALYTICAL CHEMISTRY,2017,89(1):877-885. |
| APA | Li, Zhucui.,Li, Yujing.,Chen, Wujiu.,Cao, Qichen.,Guo, Yufeng.,...&Shui, Wenqing.(2017).Integrating MS1 and MS2 Scans in High-Resolution Parallel Reaction Monitoring Assays for Targeted Metabolite Quantification and Dynamic C-13-Labeling Metabolism Analysis.ANALYTICAL CHEMISTRY,89(1),877-885. |
| MLA | Li, Zhucui,et al."Integrating MS1 and MS2 Scans in High-Resolution Parallel Reaction Monitoring Assays for Targeted Metabolite Quantification and Dynamic C-13-Labeling Metabolism Analysis".ANALYTICAL CHEMISTRY 89.1(2017):877-885. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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