High-level expression of improved thermo-stable alkaline xylanase variant in Pichia Pastoris through codon optimization, multiple gene insertion and high-density fermentation
文献类型：期刊论文
| 作者 | Lu, Yihong1; Fang, Cheng1; Wang, Qinhong2; Zhou, Yuling1; Zhang, Guimin1; Ma, Yanhe2 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2016-11-29 |
| 卷号 | 6 |
| 英文摘要 | In paper industry, xylanases are used to increase the pulp properties in bleaching process as its ecofriendly nature. The xylanases activity is hindered by high temperature and alkaline conditions with high enzyme production cost in the paper industry. Here, XynHB, an alkaline stable xylanase from Bacillus pumilus HBP8 was mutated at N188A to XynHBN188A. Expressed mutant in E. coli showed 1.5-fold higher xylanase activity than XynHB at 60 degrees C. The mutant expressed in Pichia pastoris was glycosylated, remained stable for 30 min at 60 degrees C. XynHBN188A optimized based on codon usage bias for P. pastoris (xynHBN188As) showed an increase of 39.5% enzyme activity. The strain Y16 forming the largest hydrolysis halo in the xylan plate was used in shake flask experiments produced an enzyme activity of 6,403 U/ml. The Y16 strain had 9 copies of the recombinant xynHBN188As gene in the genome revealed by qPCR. The enzymatic activity increased to 48,241 U/ml in a 5 L fermentor. Supplement of 15 U/g xylanase enhanced the brightness of paper products by 2% in bleaching experiment, and thereby improved the tensile strength and burst factor by 13% and 6.5%, respectively. XynHBN188As has a great potential in paper industries. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | TERMINAL DISULFIDE BRIDGE ; INDUSTRIAL APPLICATIONS ; MICROBIAL XYLANASES ; BACILLUS SP ; HETEROLOGOUS EXPRESSION ; MOLECULAR-CLONING ; HYDROGEN-BONDS ; THERMOSTABILITY ; PULP ; STABILITY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000388998500001 |
| 源URL | [http://124.16.173.210/handle/834782/2290]  |
| 专题 | 天津工业生物技术研究所_进化代谢工程和分子进化研究组 王钦宏_期刊论文 |
| 作者单位 | 1.Hubei Univ, Hubei Collaborat Innovat Ctr Green Transformat Bi, Coll Life Sci, Wuhan 430062, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Yihong,Fang, Cheng,Wang, Qinhong,et al. High-level expression of improved thermo-stable alkaline xylanase variant in Pichia Pastoris through codon optimization, multiple gene insertion and high-density fermentation[J]. SCIENTIFIC REPORTS,2016,6. |
| APA | Lu, Yihong,Fang, Cheng,Wang, Qinhong,Zhou, Yuling,Zhang, Guimin,&Ma, Yanhe.(2016).High-level expression of improved thermo-stable alkaline xylanase variant in Pichia Pastoris through codon optimization, multiple gene insertion and high-density fermentation.SCIENTIFIC REPORTS,6. |
| MLA | Lu, Yihong,et al."High-level expression of improved thermo-stable alkaline xylanase variant in Pichia Pastoris through codon optimization, multiple gene insertion and high-density fermentation".SCIENTIFIC REPORTS 6(2016). |
入库方式： OAI收割
来源：天津工业生物技术研究所
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