High-level expression of a ZEN-detoxifying gene by codon optimization and biobrick in Pichia pastoris
文献类型：期刊论文
| 作者 | Xiang, La1; Wang, Qinhong2; Zhou, Yuling1; Yin, Lifeng1; Zhang, Guimin1; Ma, Yanhe2 |
| 刊名 | MICROBIOLOGICAL RESEARCH
 |
| 出版日期 | 2016 |
| 卷号 | 193页码:48-56 |
| 关键词 | Zearalenone degradation Biobrick Protein expression High-density fermentation |
| 英文摘要 | The mycotoxin zearalenone (ZEN) can be degraded by a lactone hydrolase ZHD, which was derived from Gliocladium roseum. Here, based on the native ZHD encoding gene zhd101, a codon optimized zhd gene was synthesized, which was used for high expression of ZHD in Pichia pastoris GS115. Meanwhile, to further improve the expression of recombinant ZHD, the plasmids containing 1 to 4 copies of the zhd expression cassette were constructed, respectively, using the biobrick method. The protein expression in the recombinant P. pastoris X3c, which was transformed with the plasmid containing 3 copies of zhd expression cassette, was the highest. In addition, the enzymatic activity of ZHD against ZEN was defined for the first time based on a standard curve of peak area vs ZEN concentration. The ZEN degradation activity of ZHD from shake flask fermentation was calculated as 22.5 U/mL with the specific activity of 4976.5 U/mg. Furthermore, the high-density fermentation of P. pastoris X3c strain was also performed in 5 L fermenter. The maximum enzyme activity of the supernatant was 150.1 U/mL, which were 6.7-fold higher than that of the shake flask fermentation. (C) 2016 Published by Elsevier GmbH. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Microbiology |
| 研究领域[WOS] | Microbiology |
| 关键词[WOS] | ZEARALENONE ; DETOXIFICATION ; MYCOTOXIN ; CORN ; METABOLISM ; STABILITY ; COMPOUND ; FOOD |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000388247400006 |
| 源URL | [http://124.16.173.210/handle/834782/2293]  |
| 专题 | 天津工业生物技术研究所_进化代谢工程和分子进化研究组 王钦宏_期刊论文 |
| 作者单位 | 1.Hubei Univ, Coll Life Sci, Hubei Collaborat Innovat Ctr Green Transformat Bi, Wuhan 430062, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiang, La,Wang, Qinhong,Zhou, Yuling,et al. High-level expression of a ZEN-detoxifying gene by codon optimization and biobrick in Pichia pastoris[J]. MICROBIOLOGICAL RESEARCH,2016,193:48-56. |
| APA | Xiang, La,Wang, Qinhong,Zhou, Yuling,Yin, Lifeng,Zhang, Guimin,&Ma, Yanhe.(2016).High-level expression of a ZEN-detoxifying gene by codon optimization and biobrick in Pichia pastoris.MICROBIOLOGICAL RESEARCH,193,48-56. |
| MLA | Xiang, La,et al."High-level expression of a ZEN-detoxifying gene by codon optimization and biobrick in Pichia pastoris".MICROBIOLOGICAL RESEARCH 193(2016):48-56. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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