Efficient Biosynthesis of (R)- or (S)-2-Hydroxybutyrate from l-Threonine through a Synthetic Biology Approach
文献类型：期刊论文
| 作者 | Yao, Peiyuan1,2; Cui, Yunfeng1,2; Yu, Shanshan1,2; Du, Yuncheng1,2; Feng, Jinhui1,2; Wu, Qiaqing1,2; Zhu, Dunming1,2 |
| 刊名 | ADVANCED SYNTHESIS & CATALYSIS
 |
| 出版日期 | 2016-09-15 |
| 卷号 | 358期号:18页码:2923-2928 |
| 关键词 | biotransformations enzyme catalysis (R)-2-hydroxybutyric acid (S)-2-hydroxybutyric acid multi-enzyme cascade reaction |
| 英文摘要 | An efficient multi-enzyme cascade reaction for the synthesis of (R)- or (S)-2-hydroxybutyric acid [(R)- or (S)-2-HB] from l-threonine was developed by using recombinant Escherichia coli cells expressing separately or co-expressing l-threonine deaminase from Escherichia coli K-12 (ilvA), formate dehydrogenase (FDH) from Candida boidinii and l-lactate dehydrogenase (l-LDH) from Oryctolagus cuniculus or d-lactate dehydrogenase (d-LDH) from Staphylococcus epidermidis ATCC 12228. Up to 750mM of l-threonine were completely transformed to (R)- or (S)-2-HB in optically pure form (>99% ee) with high isolated yields. This one-pot multi-enzyme transformation provides a new practical method for the synthesis of these important optically pure compounds. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Applied ; Chemistry, Organic |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | D-LACTATE DEHYDROGENASE ; ENGINEERED ESCHERICHIA-COLI ; ACYL-TRANSFER CATALYST ; ALPHA-HYDROXY ESTERS ; ONE-POT SYNTHESIS ; KINETIC RESOLUTION ; CASCADE REACTIONS ; AMINO-ACIDS ; ENANTIOSELECTIVE REDUCTION ; FORMATE DEHYDROGENASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000383617800010 |
| 源URL | [http://124.16.173.210/handle/834782/2927]  |
| 专题 | 天津工业生物技术研究所_生物催化与绿色化工 朱敦明_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin Airport Econ Area, 32 Xi Qi Dao, Tianjin 300308, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Engn Res Ctr Biocatalyt Technol, Tianjin Airport Econ Area, 32 Xi Qi Dao, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yao, Peiyuan,Cui, Yunfeng,Yu, Shanshan,et al. Efficient Biosynthesis of (R)- or (S)-2-Hydroxybutyrate from l-Threonine through a Synthetic Biology Approach[J]. ADVANCED SYNTHESIS & CATALYSIS,2016,358(18):2923-2928. |
| APA | Yao, Peiyuan.,Cui, Yunfeng.,Yu, Shanshan.,Du, Yuncheng.,Feng, Jinhui.,...&Zhu, Dunming.(2016).Efficient Biosynthesis of (R)- or (S)-2-Hydroxybutyrate from l-Threonine through a Synthetic Biology Approach.ADVANCED SYNTHESIS & CATALYSIS,358(18),2923-2928. |
| MLA | Yao, Peiyuan,et al."Efficient Biosynthesis of (R)- or (S)-2-Hydroxybutyrate from l-Threonine through a Synthetic Biology Approach".ADVANCED SYNTHESIS & CATALYSIS 358.18(2016):2923-2928. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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