Novel Chemical Ligands to Ebola Virus and Marburg Virus Nucleoproteins Identified by Combining Affinity Mass Spectrometry and Metabolomics Approaches
文献类型：期刊论文
| 作者 | Fu, Xu1,2; Wang, Zhihua2,3; Li, Lixin2; Dong, Shishang2; Li, Zhucui4; Jiang, Zhenzuo5; Wang, Yuefei5; Shui, Wenqing6 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2016-07-12 |
| 卷号 | 6 |
| 英文摘要 | The nucleoprotein (NP) of Ebola virus (EBOV) and Marburg virus (MARV) is an essential component of the viral ribonucleoprotein complex and significantly impacts replication and transcription of the viral RNA genome. Although NP is regarded as a promising antiviral druggable target, no chemical ligands have been reported to interact with EBOV NP or MARV NP. We identified two compounds from a traditional Chinese medicine Gancao (licorice root) that can bind both NPs by combining affinity mass spectrometry and metabolomics approaches. These two ligands, 18 beta-glycyrrhetinic acid and licochalcone A, were verified by defined compound mixture screens and further characterized with individual ligand binding assays. Accompanying biophysical analyses demonstrate that binding of 18 beta-glycyrrhetinic acid to EBOV NP significantly reduces protein thermal stability, induces formation of large NP oligomers, and disrupts the critical association of viral ssRNA with NP complexes whereas the compound showed no such activity on MARV NP. Our study has revealed the substantial potential of new analytical techniques in ligand discovery from natural herb resources. In addition, identification of a chemical ligand that influences the oligomeric state and RNA-binding function of EBOV NP sheds new light on antiviral drug development. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | XANTHINE-OXIDASE ; LC-MS ; ULTRAFILTRATION ; INHIBITORS ; RNA ; GLYCYRRHIZIN ; REPLICATION ; DISEASE ; COMPLEX ; CHROMATOGRAPHY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000379389600002 |
| 源URL | [http://124.16.173.210/handle/834782/2909]  |
| 专题 | 天津工业生物技术研究所_生物质谱与定量生物学研究 水雯箐_期刊论文 |
| 作者单位 | 1.Tianjin Univ Sci Technol, Coll Biotechnol, Tianjin 300457, Peoples R China 2.Tianjin Joint Acad Biotechnol & Med, High Throughput Mol Drug Discovery Ctr, Tianjin 300457, Peoples R China 3.Nankai Univ, Coll Pharm, Tianjin 300071, Peoples R China 4.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 5.Tianjin Univ Tradit Chinese Med, Tianjin State Key Lab Modern Chinese Med, Tianjin 300193, Peoples R China 6.ShanghaiTech Univ, Human Inst, Shanghai 201210, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fu, Xu,Wang, Zhihua,Li, Lixin,et al. Novel Chemical Ligands to Ebola Virus and Marburg Virus Nucleoproteins Identified by Combining Affinity Mass Spectrometry and Metabolomics Approaches[J]. SCIENTIFIC REPORTS,2016,6. |
| APA | Fu, Xu.,Wang, Zhihua.,Li, Lixin.,Dong, Shishang.,Li, Zhucui.,...&Shui, Wenqing.(2016).Novel Chemical Ligands to Ebola Virus and Marburg Virus Nucleoproteins Identified by Combining Affinity Mass Spectrometry and Metabolomics Approaches.SCIENTIFIC REPORTS,6. |
| MLA | Fu, Xu,et al."Novel Chemical Ligands to Ebola Virus and Marburg Virus Nucleoproteins Identified by Combining Affinity Mass Spectrometry and Metabolomics Approaches".SCIENTIFIC REPORTS 6(2016). |
入库方式： OAI收割
来源：天津工业生物技术研究所
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