Coenzyme Engineering of a Hyperthermophilic 6-Phosphogluconate Dehydrogenase from NADP(+) to NAD(+) with Its Application to Biobatteries
文献类型：期刊论文
| 作者 | Chen, Hui1; Zhu, Zhiguang2,3; Huang, Rui1; Zhang, Yi-Heng Percival1,2,3 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2016-11-02 |
| 卷号 | 6 |
| 英文摘要 | Engineering the coenzyme specificity of redox enzymes plays an important role in metabolic engineering, synthetic biology, and biocatalysis, but it has rarely been applied to bioelectrochemistry. Here we develop a rational design strategy to change the coenzyme specificity of 6-phosphogluconate dehydrogenase (6PGDH) from a hyperthermophilic bacterium Thermotoga maritima from its natural coenzyme NADP(+) to NAD(+). Through amino acid-sequence alignment of NADP(+)-and NAD(+)-preferred 6PGDH enzymes and computer-aided substrate-coenzyme docking, the key amino acid residues responsible for binding the phosphate group of NADP(+) were identified. Four mutants were obtained via site-directed mutagenesis. The best mutant N32E/R33I/T34I exhibited a x 6.4 x 10(4)-fold reversal of the coenzyme selectivity from NADP(+) to NAD(+). The maximum power density and current density of the biobattery catalyzed by the mutant were 0.135 mW cm(-2) and 0.255 mA cm(-2), similar to 25% higher than those obtained from the wide-type 6PGDH-based biobattery at the room temperature. By using this 6PGDH mutant, the optimal temperature of running the biobattery was as high as 65 degrees C, leading to a high power density of 1.75 mW cm(-2). This study demonstrates coenzyme engineering of a hyperthermophilic 6PGDH and its application to high-temperature biobatteries. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | KETOL-ACID REDUCTOISOMERASE ; ENZYMATIC BIOFUEL CELLS ; ALCOHOL-DEHYDROGENASE ; ESCHERICHIA-COLI ; COFACTOR ; SPECIFICITY ; GLUCOSE ; DESIGN ; NADH ; BIOCATALYSIS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000387250000001 |
| 源URL | [http://124.16.173.210/handle/834782/2302]  |
| 专题 | 天津工业生物技术研究所_体外合成生物学研究组 张以恒_期刊论文 |
| 作者单位 | 1.Virginia Tech, Biol Syst Engn Dept, 304 Seitz Hall, Blacksburg, VA 24061 USA 2.Cell Free Bioinnovat Inc, 1800 Kraft Dr,Suite 222, Blacksburg, VA 24060 USA 3.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Airport Econ Area, 32 West 7th Ave, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Hui,Zhu, Zhiguang,Huang, Rui,et al. Coenzyme Engineering of a Hyperthermophilic 6-Phosphogluconate Dehydrogenase from NADP(+) to NAD(+) with Its Application to Biobatteries[J]. SCIENTIFIC REPORTS,2016,6. |
| APA | Chen, Hui,Zhu, Zhiguang,Huang, Rui,&Zhang, Yi-Heng Percival.(2016).Coenzyme Engineering of a Hyperthermophilic 6-Phosphogluconate Dehydrogenase from NADP(+) to NAD(+) with Its Application to Biobatteries.SCIENTIFIC REPORTS,6. |
| MLA | Chen, Hui,et al."Coenzyme Engineering of a Hyperthermophilic 6-Phosphogluconate Dehydrogenase from NADP(+) to NAD(+) with Its Application to Biobatteries".SCIENTIFIC REPORTS 6(2016). |
入库方式： OAI收割
来源：天津工业生物技术研究所
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