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Biosynthesis of D-Xylulose 5-Phosphate From D-Xylose and Polyphosphate Through a Minimized Two-Enzyme Cascade

文献类型：期刊论文

作者Kim, Jae-Eung1; Zhang, Y-H. Percival1,2,3,4
刊名BIOTECHNOLOGY AND BIOENGINEERING
出版日期2016-02-01
卷号113期号:2页码:275-282
关键词sugar phosphate D-xylulose 5-phosphate synthetic enzymatic pathway in vitro synthetic biosystem xylose ATP regeneration
英文摘要Sugar phosphates cannot be produced easily by microbial fermentation because negatively-charged compounds cannot be secreted across intact cell membrane. D-xylulose 5-phosphate (Xu5P), a very expensive sugar phosphate, was synthesized from D-xylose and polyphosphate catalyzed by enzyme cascades in one pot. The synthetic enzymatic pathway comprised of xylose isomerase and xylulokinase was designed to produce Xu5P, along with a third enzyme, polyphosphate kinase, responsible for in site ATP regeneration. Due to the promiscuous activity of the ATP-based xylulokinase from a hyperthermophilic bacterium Thermotoga maritima on polyphosphate, the number of enzymes in the pathway was minimized to two without polyphosphate kinase. The reactions catalyzed by the two-enzyme and three-enzyme pathways were compared for Xu5P production, and the reaction conditions were optimized by examining effects of reaction temperature, enzyme ratio and substrate concentration. The optimized two-enzyme system produced 32mM Xu5P from 50mM xylose and polyphosphate after 36 h at 45 degrees C. Biosynthesis of less costly Xu5P from D-xylose and polyphosphate could be highly feasible via this minimized two-enzyme pathway. (C) 2015 Wiley Periodicals, Inc.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]ATP REGENERATION ; AMP PHOSPHOTRANSFERASE ; COFACTOR REGENERATION ; HYDROGEN-PRODUCTION ; ESCHERICHIA-COLI ; HIGH-YIELD ; SYSTEM ; KINASE ; TRANSFORMATION ; TRANSKETOLASE
收录类别SCI
语种英语
WOS记录号WOS:000368188600004
源URL[http://124.16.173.210/handle/834782/2892]  
专题天津工业生物技术研究所_体外合成生物学研究组 张以恒_期刊论文
作者单位1.Virginia Tech, Biol Syst Engn Dept, Blacksburg, VA 24061 USA
2.Cell Free Bioinnovat Inc, Blacksburg, VA USA
3.Virginia Tech, Inst Crit Technol & Appl Sci ICTAS, Blacksburg, VA 24061 USA
4.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin, Peoples R China
推荐引用方式
GB/T 7714		 Kim, Jae-Eung,Zhang, Y-H. Percival. Biosynthesis of D-Xylulose 5-Phosphate From D-Xylose and Polyphosphate Through a Minimized Two-Enzyme Cascade[J]. BIOTECHNOLOGY AND BIOENGINEERING,2016,113(2):275-282.
APA Kim, Jae-Eung,&Zhang, Y-H. Percival.(2016).Biosynthesis of D-Xylulose 5-Phosphate From D-Xylose and Polyphosphate Through a Minimized Two-Enzyme Cascade.BIOTECHNOLOGY AND BIOENGINEERING,113(2),275-282.
MLA Kim, Jae-Eung,et al."Biosynthesis of D-Xylulose 5-Phosphate From D-Xylose and Polyphosphate Through a Minimized Two-Enzyme Cascade".BIOTECHNOLOGY AND BIOENGINEERING 113.2(2016):275-282.

入库方式： OAI收割

来源：天津工业生物技术研究所

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