De novo biosynthesis of Gastrodin in Escherichia coli
文献类型：期刊论文
| 作者 | Bai, Yanfen1,2,3; Yin, Hua1,2; Bi, Huiping1,2; Zhuang, Yibin1,2; Liu, Tao1,2; Ma, Yanhe1 |
| 刊名 | METABOLIC ENGINEERING
 |
| 出版日期 | 2016-05-01 |
| 卷号 | 35页码:138-147 |
| 关键词 | Gastrodin Chorismate Metabolic engineering Glycosyltransferase Directed evolution Escherichia coli |
| 英文摘要 | Gastrodin, a phenolic glycoside, is the key ingredient of Gastrodia elata, a notable herbal plant that has been used to treat various conditions in oriental countries for centuries. Gastrodin is extensively used clinically for its sedative, hypnotic, anticonvulsive and neuroprotective properties in China. Gastrodin is usually produced by plant extraction or chemical synthesis, which has many disadvantages. Herein, we report unprecedented microbial synthesis of gastrodin via an artificial pathway. A Nocardia carboxylic acid reductase, endogenous alcohol dehydrogenases and a Rhodiola glycosyltransferase UGT73B6 transformed 4-hydroxybenzoic acid, an intermediate of ubiquinone biosynthesis, into gastrodin in Escherichia coli. Pathway genes were overexpressed to enhance metabolic flux toward precursor 4-hydroxybenzyl alcohol. Furthermore, the catalytic properties of the UGT73B6 toward phenolic alcohols were improved through directed evolution. The finally engineered strain produced 545 mg l(-1) gastrodin in 48 h. This work creates a new route to produce gastrodin, instead of plant extractions and chemical synthesis. (C) 2016 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | RADICAL SCAVENGING ACTIVITIES ; PHENOLIC CONSTITUENTS ; SUBSTRATE-SPECIFICITY ; DIRECTED EVOLUTION ; VANILLA-PLANIFOLIA ; ELATA ; ACID ; GLYCOSYLTRANSFERASE ; YIELD ; PRODUCTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000373167900016 |
| 源URL | [http://124.16.173.210/handle/834782/2235]  |
| 专题 | 天津工业生物技术研究所_天然化合物合成生物学实验室 刘涛_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 2.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bai, Yanfen,Yin, Hua,Bi, Huiping,et al. De novo biosynthesis of Gastrodin in Escherichia coli[J]. METABOLIC ENGINEERING,2016,35:138-147. |
| APA | Bai, Yanfen,Yin, Hua,Bi, Huiping,Zhuang, Yibin,Liu, Tao,&Ma, Yanhe.(2016).De novo biosynthesis of Gastrodin in Escherichia coli.METABOLIC ENGINEERING,35,138-147. |
| MLA | Bai, Yanfen,et al."De novo biosynthesis of Gastrodin in Escherichia coli".METABOLIC ENGINEERING 35(2016):138-147. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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