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Improving isobutanol production in metabolically engineered Escherichia coli by co-producing ethanol and modulation of pentose phosphate pathway

文献类型：期刊论文

作者Liu, Zichun1,2,3; Liu, Pingping2,3; Xiao, Dongguang1; Zhang, Xueli2,3
刊名JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
出版日期2016-06-01
卷号43期号:6页码:851-860
关键词Isobutanol Ethanol Co-producing Escherichia coli NADPH
英文摘要Redox imbalance has been regarded as the key limitation for anaerobic isobutanol production in metabolically engineered Escherichia coli strains. In this work, the ethanol synthetic pathway was recruited to solve the NADH redundant problem while the pentose phosphate pathway was modulated to solve the NADPH deficient problem for anaerobic isobutanol production. Recruiting the ethanol synthetic pathway in strain AS108 decreased isobutanol yield from 0.66 to 0.29 mol/mol glucose. It was found that there was a negative correlation between aldehyde/alcohol dehydrogenase (AdhE) activity and isobutanol production. Decreasing AdhE activity increased isobutanol yield from 0.29 to 0.6 mol/mol. On the other hand, modulation of the glucose 6-phosphate dehydrogenase gene of the pentose phosphate pathway increased isobutanol yield from 0.29 to 0.41 mol/mol. Combination of these two strategies had a synergistic effect on improving isobutanol production. Isobutanol titer and yield of the best strain ZL021 were 53 mM and 0.74 mol/mol, which were 51 % and 12 % higher than the starting strain AS108, respectively. The total alcohol yield of strain ZL021 was 0.81 mol/mol, which was 23 % higher than strain AS108.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]SACCHAROMYCES-CEREVISIAE ; SUCCINATE PRODUCTION ; DEHYDROGENASE ; BIOSYNTHESIS ; BIOFUELS ; GENE ; ALCOHOLS
收录类别SCI
语种英语
WOS记录号WOS:000376930500012
源URL[http://124.16.173.210/handle/834782/2897]  
专题天津工业生物技术研究所_微生物代谢工程 张学礼_期刊论文
作者单位1.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin, Peoples R China
2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin, Peoples R China
3.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin, Peoples R China
推荐引用方式
GB/T 7714		 Liu, Zichun,Liu, Pingping,Xiao, Dongguang,et al. Improving isobutanol production in metabolically engineered Escherichia coli by co-producing ethanol and modulation of pentose phosphate pathway[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2016,43(6):851-860.
APA Liu, Zichun,Liu, Pingping,Xiao, Dongguang,&Zhang, Xueli.(2016).Improving isobutanol production in metabolically engineered Escherichia coli by co-producing ethanol and modulation of pentose phosphate pathway.JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,43(6),851-860.
MLA Liu, Zichun,et al."Improving isobutanol production in metabolically engineered Escherichia coli by co-producing ethanol and modulation of pentose phosphate pathway".JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 43.6(2016):851-860.

入库方式： OAI收割

来源：天津工业生物技术研究所

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