Use of nonimmobilized enzymes and mediators achieved high power densities in closed biobatteries
文献类型：期刊论文
| 作者 | Zhu, Zhiguang1; Zhang, Y. -H. Percival1,2,3 |
| 刊名 | ENERGY SCIENCE & ENGINEERING
 |
| 出版日期 | 2015-09-01 |
| 卷号 | 3期号:5页码:490-497 |
| 关键词 | Biobattery enzymatic fuel cell enzyme immobilization nonimmobilized mediator thermostable enzyme |
| 英文摘要 | The immobilization of redox enzymes on the surface of electrodes is a typical practice for enzymatic fuel cells (EFCs) but enzyme immobilization usually results in low retaining enzymatic activities and low power densities. Here, we investigated an alternative solution - the use of nonimmobilized thermostable enzymes (e.g., NAD-based glucose 6-phosphate dehydrogenase and flavin-containing diaphorase) and a nonimmobilized mediator (i.e., benzyl viologen or 9,10-anthraquinone-2,7-disulphonic acid, AQDS) for achieving high power densities from glucose 6-phosphate in closed EFCs (called biobatteries). At the same enzyme loading, power densities of biobatteries increased in an order from the case of immobilized enzymes and immobilized vitamin K-3 to the case of nonimmobilized enzymes and immobilized vitamin K-3 to the case of nonimmobilized enzymes and nonimmobilized AQDS. The maximum power density of the biobattery based on nonimmobilized enzymes and AQDS was 1.1 mW cm(-2) of anode at room temperature, 34-times of the biobattery based on immobilized enzymes and immobilized vitamin K-3. When enzyme loading was increased by another 10-fold, the highest power density of biobattery was increased to 2.4 mW cm(-2) at 37 degrees C. The biobattery based on nonimmobilized enzymes and AQDS retained 60% of its initial current density after running for 2 h. When repeatedly being used for 6 rounds, the biobattery had 35% of its initial current density after 12 days. Our results suggest that closed biobatteries equipped with nonimmobilized thermostable enzymes and mediators feature simple system configuration and have high power densities. |
| WOS标题词 | Science & Technology ; Technology |
| 类目[WOS] | Energy & Fuels |
| 研究领域[WOS] | Energy & Fuels |
| 关键词[WOS] | DIRECT ELECTRON-TRANSFER ; NICOTINAMIDE ADENINE-DINUCLEOTIDE ; ENZYMATIC BIOFUEL CELL ; MICROBIAL FUEL-CELL ; GLUCOSE-OXIDASE ; TOLUIDINE BLUE ; MODIFIED GRAPHITE ; CARBON NANOTUBES ; OXIDATION ; DEHYDROGENASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000374440800008 |
| 源URL | [http://124.16.173.210/handle/834782/2248]  |
| 专题 | 天津工业生物技术研究所_微生物群落及应用生态功能实验室--黄志勇_期刊论文 |
| 作者单位 | 1.Cell Free Bioinnovat Inc, 1800 Kraft Dr,Suite 222, Blacksburg, VA 24060 USA 2.Virginia Polytech Inst & State Univ, Dept Biol Syst Engn, 304 Seitz Hall, Blacksburg, VA 24061 USA 3.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Airport Econ Area, 32 West 7th Ave, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Zhiguang,Zhang, Y. -H. Percival. Use of nonimmobilized enzymes and mediators achieved high power densities in closed biobatteries[J]. ENERGY SCIENCE & ENGINEERING,2015,3(5):490-497. |
| APA | Zhu, Zhiguang,&Zhang, Y. -H. Percival.(2015).Use of nonimmobilized enzymes and mediators achieved high power densities in closed biobatteries.ENERGY SCIENCE & ENGINEERING,3(5),490-497. |
| MLA | Zhu, Zhiguang,et al."Use of nonimmobilized enzymes and mediators achieved high power densities in closed biobatteries".ENERGY SCIENCE & ENGINEERING 3.5(2015):490-497. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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