Production of 5-aminolevulinic acid by cell free multi-enzyme catalysis
文献类型：期刊论文
| 作者 | Meng, Qunglong1,2; Zhang, Yanfei1; Ju, Xiaozhi1; Ma, Chunling1; Ma, Hongwu1; Chen, Jiuzhou1; Zheng, Ping1; Sun, Jibin1; Zhu, Jun1; Ma, Yanhe1 |
| 刊名 | JOURNAL OF BIOTECHNOLOGY
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| 出版日期 | 2016-05-20 |
| 卷号 | 226页码:8-13 |
| 关键词 | 5-Aminolevulinic acid 5-Aminolevulinic acid synthase ATP regeneration Cell free production Fed-batch |
| 英文摘要 | 5-Aminolevulinic acid (ALA) is the precursor for the biosynthesis of tetrapyrroles and has broad agricultural and medical applications. Currently ALA is mainly produced by chemical synthesis and microbial fermentation. Cell free multi-enzyme catalysis is a promising method for producing high value chemicals. Here we reported our work on developing a cell free process for ALA production using thermostable enzymes. Cheap substrates (succinate and glycine) were used for ALA synthesis by two enzymes: 5-aminolevulinic acid synthase (ALAS) from Laceyella sacchari (LS-ALAS) and succinyl-CoA synthase (Suc) from Escherichia coli. ATP was regenerated by polyphosphate kinase (Ppk) using polyphosphate as the substrate. Succinate was added into the reaction system in a fed-batch mode to avoid its inhibition effect on Suc. After reaction for 160 min, ALA concentration was increased to 5.4 mM. This is the first reported work on developing the cell free process for ALA production. Through further process and enzyme optimization the cell free process could be an effective and economic way for ALA production. (C) 2016 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | SUCCINYL-COA SYNTHETASE ; ESCHERICHIA-COLI ; ATP-REGENERATION ; POLYPHOSPHATE KINASE ; HEME-BIOSYNTHESIS ; PATHWAY ; OPTIMIZATION ; ENZYMES ; GENE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000375121500002 |
| 源URL | [http://124.16.173.210/handle/834782/2258]  |
| 专题 | 天津工业生物技术研究所_系统与合成生物技术实验室 孙际宾_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, 32 West 7th Ave,Tianjin Airport Econ Area, Tianjin 300308, Peoples R China 2.Tianjin Univ, Collaborat Innovat Ctr Chem & Engn Tianjin, Sch Chem Engn & Technol, Key Lab Syst Bioengn,SynBio Res Platform,Minist E, 92 Weijin Rd, Tianjin 300072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Meng, Qunglong,Zhang, Yanfei,Ju, Xiaozhi,et al. Production of 5-aminolevulinic acid by cell free multi-enzyme catalysis[J]. JOURNAL OF BIOTECHNOLOGY,2016,226:8-13. |
| APA | Meng, Qunglong.,Zhang, Yanfei.,Ju, Xiaozhi.,Ma, Chunling.,Ma, Hongwu.,...&Chen, Tao.(2016).Production of 5-aminolevulinic acid by cell free multi-enzyme catalysis.JOURNAL OF BIOTECHNOLOGY,226,8-13. |
| MLA | Meng, Qunglong,et al."Production of 5-aminolevulinic acid by cell free multi-enzyme catalysis".JOURNAL OF BIOTECHNOLOGY 226(2016):8-13. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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