Promoter Screening from Bacillus subtilis in Various Conditions Hunting for Synthetic Biology and Industrial Applications
文献类型：期刊论文
| 作者 | Song, Yafeng1,2; Nikoloff, Jonas M.1; Fu, Gang1,2; Chen, Jingqi1,2; Li, Qinggang1,2; Xie, Nengzhong3,4; Zheng, Ping1,2; Sun, Jibin1,2; Zhang, Dawei1,2 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2016-07-05 |
| 卷号 | 11期号:7 |
| 英文摘要 | The use of Bacillus subtilis in synthetic biology and metabolic engineering is highly desirable to take advantage of the unique metabolic pathways present in this organism. To do this, an evaluation of B. subtilis' intrinsic biological parts is required to determine the best strategies to accurately regulate metabolic circuits and expression of target proteins. The strengths of promoter candidates were evaluated by measuring relative fluorescence units of a green fluorescent protein reporter, integrated into B. subtilis' chromosome. A total of 84 predicted promoter sequences located upstream of different classes of proteins including heat shock proteins, cell-envelope proteins, and proteins resistant against toxic metals (based on similarity) and other kinds of genes were tested. The expression levels measured ranged from 0.0023 to 4.53-fold of the activity of the well-characterized strong promoter P43. No significant shifts were observed when strains, carrying different promoter candidates, were cultured at high temperature or in media with ethanol, but some strains showed increased activity when cultured under high osmotic pressure. Randomly selected promoter candidates were tested and found to activate transcription of thermostable beta-galactosidase (bgaB) at a similar level, implying the ability of these sequences to function as promoter elements in multiple genetic contexts. In addition, selected promoters elevated the final production of both cytoplasmic bgaB and secreted protein alpha-amylase to about fourfold and twofold, respectively. The generated data allows a deeper understanding of B. subtilis' metabolism and will facilitate future work to develop this organism for synthetic biology. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | ESCHERICHIA-COLI STRAIN ; GENE-EXPRESSION ; SACCHAROMYCES-CEREVISIAE ; RIBOFLAVIN PRODUCTION ; RNA-POLYMERASE ; SYSTEMS ; DESIGN ; BIOSYNTHESIS ; ADAPTATION ; STRESS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000379798700032 |
| 源URL | [http://124.16.173.210/handle/834782/2913]  |
| 专题 | 天津工业生物技术研究所_系统与合成生物技术实验室 孙际宾_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 2.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China 3.Natl Engn Res Ctr Nonfood Biorefinery, State Key Lab Nonfood Biomass Energy & Enzyme Tec, Nanning 5300074, Peoples R China 4.Guangxi Acad Sci, Guangxi Biomass Ind Engn Inst, Nanning 530007, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Yafeng,Nikoloff, Jonas M.,Fu, Gang,et al. Promoter Screening from Bacillus subtilis in Various Conditions Hunting for Synthetic Biology and Industrial Applications[J]. PLOS ONE,2016,11(7). |
| APA | Song, Yafeng.,Nikoloff, Jonas M..,Fu, Gang.,Chen, Jingqi.,Li, Qinggang.,...&Zhang, Dawei.(2016).Promoter Screening from Bacillus subtilis in Various Conditions Hunting for Synthetic Biology and Industrial Applications.PLOS ONE,11(7). |
| MLA | Song, Yafeng,et al."Promoter Screening from Bacillus subtilis in Various Conditions Hunting for Synthetic Biology and Industrial Applications".PLOS ONE 11.7(2016). |
入库方式： OAI收割
来源：天津工业生物技术研究所
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