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Evolving the L-lysine high-producing strain of Escherichia coli using a newly developed high-throughput screening method

文献类型：期刊论文

作者Wang, Yan1,2,3; Li, Qinggang2,3; Zheng, Ping2,3; Guo, Yanmei3; Wang, Lixian3; Zhang, Tongcun1; Sun, Jibin2,3; Ma, Yanhe3
刊名JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
出版日期2016-09-01
卷号43期号:9页码:1227-1235
关键词Lysine production Escherichia coli Lysine biosensor High-throughput screening Strain evolution
英文摘要This study provided a new method which applied a selected L-lysine-inducible promoter for evolving lysine industrial strains of E. coli. According to the intracellular levels of the enhanced green fluorescent protein (EGFP) whose expression was controlled by the promoter, 186 strains were preliminarily selected using fluorescence-activated cell sorting from a 10-million-mutant library generated from a L-lysine high-producing E. coli strain. By subsequent multiple parameter evaluation of the 186 selected strains according to the concentration and the yield of lysine, the productivity per unit of cell in 96-deep-well blocks, two mutants MU-1 and MU-2 were obtained. They produced 136.51 +/- 1.55 and 133.2 9 +/- 1.42 g/L of lysine, respectively, in 5-L jars. Compared with the lysine concentration and the yield of the original strain, those of strain MU-1 improved by 21.00 and 9.05 %, respectively, and those of strain MU-2 improved by 18.14 and 10.41 %, respectively. The mutant selection and evaluation system newly established in our study should be useful for continuous improvement of the current E. coli strains in the lysine industry.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology
关键词[WOS]CORYNEBACTERIUM-GLUTAMICUM ; METABOLITE CONCENTRATIONS ; BINDING-SITES ; EVOLUTION ; GENOME ; IMPROVEMENT ; EXPRESSION ; BIOSENSORS ; BACTERIA ; GENE
收录类别SCI
语种英语
WOS记录号WOS:000384417500004
源URL[http://124.16.173.210/handle/834782/2937]  
专题天津工业生物技术研究所_系统与合成生物技术实验室 孙际宾_期刊论文
作者单位1.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin, Peoples R China
2.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin, Peoples R China
3.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Yan,Li, Qinggang,Zheng, Ping,et al. Evolving the L-lysine high-producing strain of Escherichia coli using a newly developed high-throughput screening method[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2016,43(9):1227-1235.
APA Wang, Yan.,Li, Qinggang.,Zheng, Ping.,Guo, Yanmei.,Wang, Lixian.,...&Ma, Yanhe.(2016).Evolving the L-lysine high-producing strain of Escherichia coli using a newly developed high-throughput screening method.JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,43(9),1227-1235.
MLA Wang, Yan,et al."Evolving the L-lysine high-producing strain of Escherichia coli using a newly developed high-throughput screening method".JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 43.9(2016):1227-1235.

入库方式： OAI收割

来源：天津工业生物技术研究所

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