A simple assay for determining activities of phosphopentomutase from a hyperthermophilic bacterium Thermotoga maritima
文献类型：期刊论文
| 作者 | Moustafa, Hanan M. A.1,2; Zaghloul, Taha I.2; Zhang, Y-H. Percival1,3 |
| 刊名 | ANALYTICAL BIOCHEMISTRY
 |
| 出版日期 | 2016-05-15 |
| 卷号 | 501页码:75-81 |
| 关键词 | Biocatalysis Enzymatic assay Phosphopentomutase Thermophilic enzyme Thermotoga maritima |
| 英文摘要 | Phosphopentomutase (PPM) catalyzes the interconversion of alpha-D-(deoxy)-ribose 1-phosphate and alpha-D-(deoxy)-ribose 5-phosphate. We developed a coupled or uncoupled enzymatic assay with an enzyme nucleoside phosphorylase for determining PPM activities on D-ribose 5-phosphate at a broad temperature range from 30 to 90 degrees C. This assay not only is simple and highly sensitive but also does not require any costly special instrument. Via this technology, an open reading frame TM0167 from a thermophilic bacterium Thermotoga maritima putatively encoding PPM was cloned. The recombinant PPM was overexpressed in Escherichia coli Rosetta. This enzyme has the highest activity at 90 degrees C. MnCl2 (0.1 mM) and 50 mu M alpha-D-glucose 1,6-bisphosphate are cofactors. The kinetic parameters of K-m and k(cat) are 1.2 mM and 185 s(-1) at 90 degrees C, respectively. The enzyme has a half-life time of up to 156 min at 90 degrees C. This enzyme is the most active and thermostable PPM reported to date. (C) 2016 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry |
| 关键词[WOS] | ENZYME COMPLEX ; PHOSPHODEOXYRIBOMUTASE ; PURIFICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000374204300011 |
| 源URL | [http://124.16.173.210/handle/834782/2254]  |
| 专题 | 天津工业生物技术研究所_总体研究部_期刊论文 |
| 作者单位 | 1.Virginia Tech, Dept Biol Syst Engn, Blacksburg, VA 24061 USA 2.Univ Alexandria, Inst Grad Studies & Res, Dept Biotechnol, Alexandria 21526, Egypt 3.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Airport Econ Area, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Moustafa, Hanan M. A.,Zaghloul, Taha I.,Zhang, Y-H. Percival. A simple assay for determining activities of phosphopentomutase from a hyperthermophilic bacterium Thermotoga maritima[J]. ANALYTICAL BIOCHEMISTRY,2016,501:75-81. |
| APA | Moustafa, Hanan M. A.,Zaghloul, Taha I.,&Zhang, Y-H. Percival.(2016).A simple assay for determining activities of phosphopentomutase from a hyperthermophilic bacterium Thermotoga maritima.ANALYTICAL BIOCHEMISTRY,501,75-81. |
| MLA | Moustafa, Hanan M. A.,et al."A simple assay for determining activities of phosphopentomutase from a hyperthermophilic bacterium Thermotoga maritima".ANALYTICAL BIOCHEMISTRY 501(2016):75-81. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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