Efficient and cost-effective production of D-p-hydroxyphenylglycine by whole-cell bioconversion
文献类型：期刊论文
| 作者 | Zhang, Junli1,2; Cai, Zhen1 |
| 刊名 | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
 |
| 出版日期 | 2014-02-01 |
| 卷号 | 19期号:1页码:76-82 |
| 关键词 | D-p-hydroxyphenylglycine whole-cell bioconversion D-hydantoinase N-carbamoylase large-scale |
| 英文摘要 | D-p-hydroxyphenylglycine (D-HPG) is a widely used intermediate for the synthesis of semi-synthetic antibiotics. It can be produced from DL-5-p-hydroxyphenylhydantoin through two sequential enzymatic reactions catalyzed by D-hydantoinase and N-carbamoylase. However, the low productivity and high production cost of the current process significantly limit its industrial application. To set up an efficient and cost-effective whole-cell bioconversion process for D-HPG production, a recombinant E. coli strain was constructed by co-expressing D-hydantoinase and N-carbamoylase from Agrobacterium sp. Then a cheap medium formulation, which uses glycerol and corn steep liquor (CSL) as carbon and nitrogen sources and without addition of any foreign inducer, was developed for high level of enzyme expression. Galactose, melibiose, and raffinose in CSL were found to be capable of inducing T7 promoter. Moreover, this CSL-containing cheap medium exhibited higher expression levels than the traditional LB+IPTG medium for several different enzymes tested, indicating that this medium might be a better alternative to the commonly used LB+IPTG medium for enzyme expression under the control of a T7 promoter. Further optimization revealed that low expression temperature not only increased enzyme expression, but also stabilized the enzyme-expressing cells and the plasmids therein. Wholecell bioconversion was carried out in 55 t water containing 1.8 t substrate and the resting cells expressed in 40 t medium. The specific D-HPG productivity reached 1.68 g/h/g dry cell weight, with a molar yield of 97.8%. To the best of our knowledge, this is the highest productivity reported to date and the first description of this process using large-scale production. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | D-AMINO ACIDS ; ESCHERICHIA-COLI STRAINS ; D-HYDANTOINASE ; 5-SUBSTITUTED HYDANTOINS ; RECOMBINANT PROTEINS ; ENZYMATIC PRODUCTION ; DL-5-P-HYDROXYPHENYLHYDANTOIN ; AMIDOHYDROLASE ; PURIFICATION ; CARBAMOYLASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000333012300010 |
| 源URL | [http://124.16.173.210/handle/834782/2266]  |
| 专题 | 天津工业生物技术研究所_总体研究部_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Junli,Cai, Zhen. Efficient and cost-effective production of D-p-hydroxyphenylglycine by whole-cell bioconversion[J]. BIOTECHNOLOGY AND BIOPROCESS ENGINEERING,2014,19(1):76-82. |
| APA | Zhang, Junli,&Cai, Zhen.(2014).Efficient and cost-effective production of D-p-hydroxyphenylglycine by whole-cell bioconversion.BIOTECHNOLOGY AND BIOPROCESS ENGINEERING,19(1),76-82. |
| MLA | Zhang, Junli,et al."Efficient and cost-effective production of D-p-hydroxyphenylglycine by whole-cell bioconversion".BIOTECHNOLOGY AND BIOPROCESS ENGINEERING 19.1(2014):76-82. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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