Production of Succinate from Acetate by Metabolically Engineered Escherichia coli
文献类型：期刊论文
| 作者 | Li, Yunjie1,4; Huang, Bing1; Wu, Hui1; Li, Zhimin1,2; Ye, Qin1; Zhang, Y-H Percival3,4 |
| 刊名 | ACS SYNTHETIC BIOLOGY
 |
| 出版日期 | 2016-11-01 |
| 卷号 | 5期号:11页码:1299-1307 |
| 关键词 | acetate succinate metabolic engineering Escherichia coli malic enzyme citrate synthase |
| 英文摘要 | Acetate, a major component of industrial biological wastewater and of lignocellulosic biomass hydrolysate, could potentially be a less costly alternative carbon source. Here we engineered Escherichia coli MG1655 strain for succinate production from acetate as the sole carbon source. Strategies of metabolic engineering included the blockage of the TCA cycle, redirection of the gluconeogenesis pathway, and enhancement of the glyoxylate shunt. The engineered strain MG03 featuring the deletion of genes: succinate dehydrogenase (sdhAB), isocitrate lyase regulator (iclR), and malic enzymes (maeB) accumulated 6.86 mM of succinate in 72 h. MG03(pTrc99a-gltA) overexpressing citrate synthase (g1tA) accumulated 16.45 mM of succinate and the yield reached 0.46 mol/mol, about 92% of the maximum theoretical yield. Resting-cell was adopted for the conversion of acetate to succinate, and the highest concentration of succinate achieved 61.7 mM. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | ACID PRODUCTION ; CORYNEBACTERIUM-GLUTAMICUM ; ACETIC-ACID ; CARBON ; GENES ; FERMENTATIONS ; STRATEGY ; METHANOL ; STRAINS ; GLUCOSE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000388428100014 |
| 源URL | [http://124.16.173.210/handle/834782/2279]  |
| 专题 | 天津工业生物技术研究所_总体研究部_期刊论文 |
| 作者单位 | 1.East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China 2.Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China 3.Virginia Tech, Dept Biol Syst Engn, 304 Seitz Hall, Blacksburg, VA 24061 USA 4.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Airport Econ Area, 32 West Seventh Ave, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yunjie,Huang, Bing,Wu, Hui,et al. Production of Succinate from Acetate by Metabolically Engineered Escherichia coli[J]. ACS SYNTHETIC BIOLOGY,2016,5(11):1299-1307. |
| APA | Li, Yunjie,Huang, Bing,Wu, Hui,Li, Zhimin,Ye, Qin,&Zhang, Y-H Percival.(2016).Production of Succinate from Acetate by Metabolically Engineered Escherichia coli.ACS SYNTHETIC BIOLOGY,5(11),1299-1307. |
| MLA | Li, Yunjie,et al."Production of Succinate from Acetate by Metabolically Engineered Escherichia coli".ACS SYNTHETIC BIOLOGY 5.11(2016):1299-1307. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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