Exceptionally High Rates of Biological Hydrogen Production by Biomimetic In Vitro Synthetic Enzymatic Pathways
文献类型：期刊论文
| 作者 | Kim, Eui-Jin1; Wu, Chang-Hao2; Adams, Michael W. W.2; Zhang, Y. -H. Percival1,3 |
| 刊名 | CHEMISTRY-A EUROPEAN JOURNAL
 |
| 出版日期 | 2016-11-01 |
| 卷号 | 22期号:45页码:16047-16051 |
| 关键词 | electron mediator electron-transport chain hydrogen production synthetic enzymatic pathway water splitting |
| 英文摘要 | Hydrogen production by water splitting energized by biomass sugars is one of the most promising technologies for distributed green H-2 production. Direct H-2 generation from NADPH, catalysed by an NADPH-dependent, soluble [NiFe]-hydrogenase (SH1) is thermodynamically unfavourable, resulting in slow volumetric productivity. We designed the biomimetic electron transport chain from NADPH to H-2 by the introduction of an oxygen-insensitive electron mediator benzyl viologen (BV) and an enzyme (NADPH rubredoxin oxidoreductase, NROR), catalysing electron transport between NADPH and BV. The H-2 generation rates using this biomimetic chain increased by approximately five-fold compared to those catalysed only by SH1. The peak volumetric H-2 productivity via the in vitro enzymatic pathway comprised of hyperthermophilic glucose 6-phosphate dehydrogenase, 6-phosphogluconolactonase, and 6-phosphogluconate dehydrogenase, NROR, and SH1 was 310 mmol H-2/Lh(-1), the highest rate yet reported. The concept of biomimetic electron transport chains could be applied to both in vitro and in vivo H-2 production biosystems and artificial photo-synthesis. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Multidisciplinary |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | PYROCOCCUS-FURIOSUS ; HYPERTHERMOPHILIC ARCHAEON ; BIOHYDROGEN PRODUCTION ; THERMOTOGA-MARITIMA ; PURIFICATION ; FERREDOXIN ; REDUCTION ; TOLERANCE ; VIOLOGEN ; NADPH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000387132800010 |
| 源URL | [http://124.16.173.210/handle/834782/2294]  |
| 专题 | 天津工业生物技术研究所_总体研究部_期刊论文 |
| 作者单位 | 1.Virginia Tech, Dept Biol Syst Engn, 304 Seitz Hall, Blacksburg, VA 24061 USA 2.Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA 3.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Airport Econ Area, 32 West 7th Ave, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Kim, Eui-Jin,Wu, Chang-Hao,Adams, Michael W. W.,et al. Exceptionally High Rates of Biological Hydrogen Production by Biomimetic In Vitro Synthetic Enzymatic Pathways[J]. CHEMISTRY-A EUROPEAN JOURNAL,2016,22(45):16047-16051. |
| APA | Kim, Eui-Jin,Wu, Chang-Hao,Adams, Michael W. W.,&Zhang, Y. -H. Percival.(2016).Exceptionally High Rates of Biological Hydrogen Production by Biomimetic In Vitro Synthetic Enzymatic Pathways.CHEMISTRY-A EUROPEAN JOURNAL,22(45),16047-16051. |
| MLA | Kim, Eui-Jin,et al."Exceptionally High Rates of Biological Hydrogen Production by Biomimetic In Vitro Synthetic Enzymatic Pathways".CHEMISTRY-A EUROPEAN JOURNAL 22.45(2016):16047-16051. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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