Convergent Regulation of Candida albicans Aft2 and Czf1 in Invasive and Opaque Filamentation
文献类型：期刊论文
| 作者 | Xu, Ning1,2; Dong, Yi-Jie1; Yu, Qi-Lin1; Zhang, Bing1; Zhang, Meng1; Jia, Chang1; Chen, Yu-Lu1; Zhang, Biao3; Xing, Lai-Jun1; Li, Ming-Chun1 |
| 刊名 | JOURNAL OF CELLULAR BIOCHEMISTRY
 |
| 出版日期 | 2015-09-01 |
| 卷号 | 116期号:9页码:1908-1918 |
| 关键词 | Candida albicans Aft2 FILAMENTOUS GROWTH WHITE-OPAQUE SWITCHING INVASIVE FILAMENTATION |
| 英文摘要 | Candida albicans is the most common fungal pathogen of mucosal infections and invasive diseases in immuno-compromised humans. The abilities of yeast-hyphal growth and white-opaque switching affect C. albicans physiology and virulence. Here, we showed that C. albicans Aft2 regulator was required for embedded filamentous growth and opaque cell-type formation. Under low-temperature matrix embedded conditions, Aft2 functioned downstream of Czf1-mediated pathway and was required for invasive filamentation. Moreover, deletion of AFT2 significantly reduced opaque cell-type formation under N-acetylglucosamine (GlcNAc) inducing conditions. Ectopic expression of CZF1 slightly increased the white-opaque switching frequency in the aft2/ mutant, but did not completely restore to wild-type levels, suggesting that Czf1 at least partially bypassed the essential requirement for Aft2 in response to opaque-inducing cues. In addition, multiple environmental cues altered AFT2 mRNA and protein levels, such as low temperature, physical environment and GlcNAc. Although the absence of Czf1 or Efg1 also increased the expression level of AFT2 gene, deletion of CZF1 remarkably reduced the stability of Aft2 protein. Furthermore, C. albicans Aft2 physically interacted with Czf1 under all tested conditions, whereas the interaction between Aft2 and Efg1 was barely detectable under embedded conditions, supporting the hypothesis that Aft2, together with Czf1, contributed to activate filamentous growth by antagonizing Efg1-mediated repression under matrix-embedded conditions. J. Cell. Biochem. 116: 1908-1918, 2015. (c) 2015 Wiley Periodicals, Inc. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | MORPHOGENETIC REGULATOR ; TRANSCRIPTION FACTOR ; GROWTH ; GENE ; EXPRESSION ; VIRULENCE ; COMMENSALISM ; PROTEINS ; FARNESOL ; EFG1P |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000358506300011 |
| 源URL | [http://124.16.173.210/handle/834782/2885]  |
| 专题 | 天津工业生物技术研究所_总体研究部_期刊论文 |
| 作者单位 | 1.Nankai Univ, Coll Life Sci, Key Lab Mol Microbiol & Technol, Minist Educ, Tianjin 300071, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 3.Tianjin Univ Tradit Chinese Med, Tianjin 300193, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Ning,Dong, Yi-Jie,Yu, Qi-Lin,et al. Convergent Regulation of Candida albicans Aft2 and Czf1 in Invasive and Opaque Filamentation[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2015,116(9):1908-1918. |
| APA | Xu, Ning.,Dong, Yi-Jie.,Yu, Qi-Lin.,Zhang, Bing.,Zhang, Meng.,...&Li, Ming-Chun.(2015).Convergent Regulation of Candida albicans Aft2 and Czf1 in Invasive and Opaque Filamentation.JOURNAL OF CELLULAR BIOCHEMISTRY,116(9),1908-1918. |
| MLA | Xu, Ning,et al."Convergent Regulation of Candida albicans Aft2 and Czf1 in Invasive and Opaque Filamentation".JOURNAL OF CELLULAR BIOCHEMISTRY 116.9(2015):1908-1918. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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