The Ccz1 mediates the autophagic clearance of damaged mitochondria in response to oxidative stress in Candida albicans
文献类型：期刊论文
| 作者 | Dong, Yijie1; Yu, Qilin1; Chen, Yulu1; Xu, Ning3; Zhao, Qiang1; Jia, Chang1; Zhang, Bing1; Zhang, Kai1; Zhang, Biao2; Xing, Laijun1 |
| 刊名 | INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
 |
| 出版日期 | 2015-12-01 |
| 卷号 | 69页码:41-51 |
| 关键词 | Candida albicans Ccz1 Autophagy Oxidative stress Virulence |
| 英文摘要 | Autophagy plays a critical role in response to numerous cellular stresses, such as nutrient deprivation, hypoxia, starvation and organelle damage. The disruption of autophagy pathway affects multiple aspects of cellular stress response. Here we for the first time identified Ccz1 as an essential component for autophagy in Candida albicans. Our experiments demonstrated that loss of CCZ1 gene led to vacuolar fragmentation and disruption of the autophagy pathway. Our results also suggested that Ccz1 functioned in oxidative stress. In the Ccz1 Delta/Delta mutant, the levels of reactive oxidative species (ROS) sharply increased under H2O2 treatment. Further studies demonstrated that breakdown of the autophagic clearance pathway led to the accumulation of oxidative stress-damaged mitochondria, and consequently elevated cellular ROS levels in the ccz1 Delta/Delta. mutant. Furthermore, deletion of CCZ1 led to a significant defect in filamentous development at both 30 degrees C and 37 degrees C. The disruption of CCZ/ gene led to decreased capacity of macrophage killing and increased sensitivity to the macrophages. In addition, the ccz1 Delta/Delta mutant exhibited attenuated virulence and decreased fungal burdens in the mouse systemic infection model, indicating that CCZ1 might provide a promising target for antifungal drugs development. In summary, our findings provide new insights into the understanding of autophagy-related gene in C. albicans. (C) 2015 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | VACUOLAR MEMBRANE PERMEABILIZATION ; SACCHAROMYCES-CEREVISIAE ; PROTEIN CCZ1P ; IRON ACQUISITION ; CELL-DEATH ; H+-ATPASE ; YEAST ; VIRULENCE ; MORPHOGENESIS ; MITOPHAGY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000367020800005 |
| 源URL | [http://124.16.173.210/handle/834782/2891]  |
| 专题 | 天津工业生物技术研究所_总体研究部_期刊论文 |
| 作者单位 | 1.Nankai Univ, Coll Life Sci, Dept Microbiol, Key Lab Mol Microbiol & Technol,Minist Educ, Tianjin 300071, Peoples R China 2.Tianjin Univ Tradit Chinese Med, Tianjin, Peoples R China 3.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dong, Yijie,Yu, Qilin,Chen, Yulu,et al. The Ccz1 mediates the autophagic clearance of damaged mitochondria in response to oxidative stress in Candida albicans[J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,2015,69:41-51. |
| APA | Dong, Yijie.,Yu, Qilin.,Chen, Yulu.,Xu, Ning.,Zhao, Qiang.,...&Li, Mingchun.(2015).The Ccz1 mediates the autophagic clearance of damaged mitochondria in response to oxidative stress in Candida albicans.INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,69,41-51. |
| MLA | Dong, Yijie,et al."The Ccz1 mediates the autophagic clearance of damaged mitochondria in response to oxidative stress in Candida albicans".INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY 69(2015):41-51. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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