Preparative and Rapid Purification of Saponins from Asparagus racemosus Root by High Performance Centrifugal Partition Chromatography
文献类型：期刊论文
| 作者 | Onlom, C (Onlom, Churanya); Yang, Y (Yang, Yi); Aisa, HA (Aisa, Haji A.) ; Woranuch, N (Woranuch, Neti); Phrompittayarat, W (Phrompittayarat, Watoo); Putalun, W (Putalun, Waraporn); Ingkaninan, K (Ingkaninan, Kornkanok)
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| 刊名 | NATURAL PRODUCT COMMUNICATIONS
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| 出版日期 | 2017 |
| 卷号 | 12期号:2页码:241-244 |
| 关键词 | Asparagus racernosus Cytotoxicity High performance centrifugal partition chromatography Saponins Carcinoma cells |
| 英文摘要 | High performance centrifugal partition chromatography (HPCPC) was applied to the rapid isolation and purification of saponin glycosides in Asparagus racernosus Willd. root. A two-phase solvent system composed of CHCl3-MeOH-water (4:4:2, v/v) in descending mode was used for the separation, yielding shatavarin IX (1) and asparacoside (2) in one step. Asparanin A (3) and shatavarin V (4) were separated by repeated HPCPC fractionation using CH2Cl2-MeOH-water (4:4:2, v/v) as the solvent system, followed by either gel-filtration or TLC. Their structures were identified by NMR spectroscopy and ESI/MS. The A. racemosus extracts and 1, 2, 3 and 4 were cytotoxic towards human hepato- and prostate-carcinoma cell lines (IC50 14-37 mu M), while primary human fibroblasts were less vulnerable (IC50 22-66 mu M), i.e., every saponin glycoside showed selectivity towards carcinoma cells compared with normal fibroblasts. HPCPC has proven rapidity to separate complex mixtures of phytochemicals yielding quantities suited to biological studies. |
| 收录类别 | SCI |
| WOS记录号 | WOS:000395229200027 |
| 源URL | [http://ir.xjipc.cas.cn/handle/365002/4750]  |
| 专题 | 新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室 新疆理化技术研究所_资源化学研究室 |
| 作者单位 | 1.Naresuan Univ, Fac Pharmaceut Sci, Dept Pharmaceut Chem & Pharmacognosy, Bioscreening Unit, Phitsanulok 65000, Thailand 2.Naresuan Univ, Ctr Excellence Innovat Chem, Phitsanulok 65000, Thailand 3.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China 4.Naresuan Univ, Fac Pharmaceut Sci, Cosmet & Nat Prod Res Unit, Phitsanulok 65000, Thailand 5.Naresuan Univ, Fac Publ Hlth, Phitsanulok 65000, Thailand 6.Khon Kaen Univ, Fac Pharmaceut Sci, Khon Kaen 40002, Thailand |
| 推荐引用方式 GB/T 7714 | Onlom, C ,Yang, Y ,Aisa, HA ,et al. Preparative and Rapid Purification of Saponins from Asparagus racemosus Root by High Performance Centrifugal Partition Chromatography[J]. NATURAL PRODUCT COMMUNICATIONS,2017,12(2):241-244. |
| APA | Onlom, C .,Yang, Y .,Aisa, HA .,Woranuch, N .,Phrompittayarat, W .,...&Ingkaninan, K .(2017).Preparative and Rapid Purification of Saponins from Asparagus racemosus Root by High Performance Centrifugal Partition Chromatography.NATURAL PRODUCT COMMUNICATIONS,12(2),241-244. |
| MLA | Onlom, C ,et al."Preparative and Rapid Purification of Saponins from Asparagus racemosus Root by High Performance Centrifugal Partition Chromatography".NATURAL PRODUCT COMMUNICATIONS 12.2(2017):241-244. |
入库方式： OAI收割
来源：新疆理化技术研究所
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