Preparation, Phytochemical Investigation, and Safety Evaluation of Chlorogenic Acid Products from Eupatorium adenophorum
文献类型：期刊论文
| 作者 | Liu, Boyan1,2; Cao, Lili1; Zhang, Lijun1,2; Yuan, Xiaofan1 ; Zhao, Bing1
|
| 刊名 | MOLECULES
 |
| 出版日期 | 2017 |
| 卷号 | 22期号:1 |
| 关键词 | Eupatorium adenophorum chlorogenic acid sesquiterpenes safety toxicity |
| ISSN号 | 1420-3049 |
| 英文摘要 | Eupatorium adenophorum is widely distributed throughout the world's tropical and temperate regions. It has become a harmful weed of crops and natural environments. Its leaves contain bioactive compounds such as chlorogenic acid and may be used as feed additives. In this study, chlorogenic acid was extracted and separated from leaves of E. adenophorum. Three chlorogenic acid products were prepared with different purities of 6.11%, 22.17%, and 96.03%. Phytochemical analysis demonstrated that the main toxins of sesquiterpenes were almost completely removed in sample preparation procedure. The three products were evaluated for safety via in vitro and in vivo toxicological studies. All the products exhibited no cytotoxic effects at a dose of 400 mu g/mL in an in vitro cell viability assay. When administered in vivo at a single dose up to 1.5 g/kg bw, all three products caused no signs or symptoms of toxicity in mice. These results encourage further exploration of extracts from E. adenophorum in feed additive application. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Organic |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | IN-VITRO ; AGERATINA-ADENOPHORA ; COUNTERCURRENT CHROMATOGRAPHY ; ESSENTIAL OILS ; CAFFEIC ACID ; HEPG2 CELLS ; SEPARATION ; HEPATOTOXICITY ; CYTOTOXICITY ; PURIFICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000395473500067 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/21994]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Div Biorefinery Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Boyan,Cao, Lili,Zhang, Lijun,et al. Preparation, Phytochemical Investigation, and Safety Evaluation of Chlorogenic Acid Products from Eupatorium adenophorum[J]. MOLECULES,2017,22(1). |
| APA | Liu, Boyan,Cao, Lili,Zhang, Lijun,Yuan, Xiaofan,&Zhao, Bing.(2017).Preparation, Phytochemical Investigation, and Safety Evaluation of Chlorogenic Acid Products from Eupatorium adenophorum.MOLECULES,22(1). |
| MLA | Liu, Boyan,et al."Preparation, Phytochemical Investigation, and Safety Evaluation of Chlorogenic Acid Products from Eupatorium adenophorum".MOLECULES 22.1(2017). |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。