High-Efficiency Secretion of beta-Mannanase in Bacillus subtilis through Protein Synthesis and Secretion Optimization
文献类型：期刊论文
| 作者 | Song, Yafeng1,2,3; Fu, Gang1,2; Dong, Huina1,2; Li, Jianjun4,5; Du, Yuguang4,5; Zhang, Dawei1,2 |
| 刊名 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
 |
| 出版日期 | 2017-03-29 |
| 卷号 | 65期号:12页码:2540-2548 |
| 关键词 | beta-mannanase Bacillus subtilis signal peptides promoters chaperones signal peptidase |
| ISSN号 | 0021-8561 |
| 英文摘要 | The manno endo-1,4-mannosidase (beta-mannanase, EC. 3.2.1.78) catalyzes the random hydrolysis of internal (1 -> 4)-beta-mannosidic linkages in the mannan polymers. A codon optimized beta-mannanase gene from Bacillus licheniformis DSM13 was expressed in Bacillus subtilis. When four Sec-dependent and two Tat-dependent signal peptide sequences cloned from B. subtilis were placed upstream of the target gene, the highest activity of P-mannanase was observed using SPlipA,A as a signal "peptide. Then a 1.25-fold activity beta-mannanase was obtained when another copy of groESL operon was inserted into the genome of host strain. Finally, five different promoters were separately used to enhance the synthesis of the target protein. The results showed that promoter P-mglv a modified maltose-inducible promoter, significantly elevated the production beta-mannanase. After 72 h of flask fermentation, the enzyme activity of P-mannanase in the supernatant when using locust bean gum as substrate reached 2207 U/mL. This work provided a promising beta-mannanase production strain in industrial application. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology |
| 研究领域[WOS] | Agriculture ; Chemistry ; Food Science & Technology |
| 关键词[WOS] | METHYL PARATHION HYDROLASE ; GRAM-POSITIVE BACTERIA ; EXTRACELLULAR PRODUCTION ; ESCHERICHIA-COLI ; ALPHA-GALACTOSIDASE ; EXPRESSION SYSTEMS ; SIGNAL PEPTIDES ; VECTORS ; TRANSFORMATION ; LICHENIFORMIS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000398247300006 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/22071]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 2.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China 3.Univ Groningen, Groningen Res Inst Pharm, Dept Chem & Pharmaceut Biol, NL-9713 AV Groningen, Netherlands 4.Natl Engn Res Ctr Biotechnol, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 5.Chinese Acad Sci, Inst Proc Engn, Key Lab Biopharmaceut Prod & Formulat Engn, PLA, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Yafeng,Fu, Gang,Dong, Huina,et al. High-Efficiency Secretion of beta-Mannanase in Bacillus subtilis through Protein Synthesis and Secretion Optimization[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2017,65(12):2540-2548. |
| APA | Song, Yafeng,Fu, Gang,Dong, Huina,Li, Jianjun,Du, Yuguang,&Zhang, Dawei.(2017).High-Efficiency Secretion of beta-Mannanase in Bacillus subtilis through Protein Synthesis and Secretion Optimization.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,65(12),2540-2548. |
| MLA | Song, Yafeng,et al."High-Efficiency Secretion of beta-Mannanase in Bacillus subtilis through Protein Synthesis and Secretion Optimization".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 65.12(2017):2540-2548. |
入库方式： OAI收割
来源：过程工程研究所
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