An insecticidal toxin from Nephila clavata spider venom
文献类型：期刊论文
| 作者 | Jin L1; Fang MQ1; Hakim MA2; Mo GX1; Lai R2,3; Yan XW[*]1; Wang YM[*]4; Yang SL[*]2; Chen MR1; Zhou CL1 |
| 刊名 | Amino Acids
 |
| 出版日期 | 2017 |
| 卷号 | **期号:**页码:published online |
| 关键词 | Toxin Spider Venom Nephila clavata Bioinsecticide |
| 通讯作者 | wangym992011@163.com ; yangsl@mail.kiz.ac.cn ; yanxw@njau.edu.cn |
| 英文摘要 | Spiders are the most successful insect predators given that they use their venom containing insecticidal peptides as biochemical weapons for preying. Due to the high specificity and potency of peptidic toxins, discoveries of insecticidal toxins from spider venom have provided an opportunity to obtain natural compounds for agricultural applications without affecting human health. In this study, a novel insecticidal toxin (μ-NPTX-Nc1a) was identified and characterized from the venom of Nephila clavata. Its primary sequence is GCNPDCTGIQCGWPRCPGGQNPVMDKCVSCCPFCPPKSAQG which was determined by automated Edman degradation, cDNA cloning, and MS/MS analysis. BLAST search indicated that Nc1a shows no similarity with known peptides or proteins, indicating that Nc1a belongs to a novel family of insecticidal peptide. Nc1a displayed inhibitory effects on NaV and KV channels in cockroach dorsal unpaired median neurons. The median lethal dose (LD50) of Nc1a on cockroach was 573 ng/g. Herein, a study that identifies a novel insecticidal toxin, which can be a potential candidate and/or template for the development of bioinsecticides, is presented. |
| 资助信息 | This work was supported by funding from the Ministry of Science and Technology of China (2013CB911304), National Science Foundation of China (331372208 and U1302221), Chinese Academy of Sciences (XDA12040209, QYZDJ-SSW-SMC012), Science and technology office of Jiangsu Province (BE2016742) and Yunnan Province (2015HA023) to R.L., National Science Foundation of China (31640071) and Chinese Academy of Sciences (XDA12020334 and Youth Innovation Promo- tion Association) to S.Y., and National Science Foundation of China (31201717), Jiangsu Province (Q0201600440) to X.W.Y. |
| 语种 | 英语 |
| 源URL | [http://159.226.149.26:8080/handle/152453/11495]  |
| 专题 | 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 昆明动物研究所_动物毒素室 |
| 作者单位 | 1.College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China 2.Key Laboratory of Animal Models and Human Disease Mechanisms, Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Kunming 650223, Yunnan, China 3.Sino-African Joint Research Center, CAS, Kunming Institute of Zoology, Kunming 650223, Yunnan, China 4.Clinical Laboratory, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, Yunnan, China 5.University of Chinese Academy of Sciences, Beijing 100009, China |
| 推荐引用方式 GB/T 7714 | Jin L,Fang MQ,Hakim MA,et al. An insecticidal toxin from Nephila clavata spider venom[J]. Amino Acids,2017,**(**):published online. |
| APA | Jin L.,Fang MQ.,Hakim MA.,Mo GX.,Lai R.,...&Ombati R.(2017).An insecticidal toxin from Nephila clavata spider venom.Amino Acids,**(**),published online. |
| MLA | Jin L,et al."An insecticidal toxin from Nephila clavata spider venom".Amino Acids **.**(2017):published online. |
入库方式： OAI收割
来源：昆明动物研究所
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