Using eDNA to detect the distribution and density of invasive crayfish in the Honghe- Hani rice terrace World Heritage site
文献类型：期刊论文
| 作者 | Cai W1,2; Ma ZX3; Zhao GG4,5; Geng YP[*]3; Yu DW1,6; Yang CY[*]1; Wang L1; Wang WZ1 |
| 刊名 | PLos One
 |
| 出版日期 | 2017 |
| 卷号 | 15期号:5页码:e0177724 |
| 其他题名 | Support was provided by the National Natural Science Foundation of China to CYY and DWY;the Ministry of Science and Technology of the People’s Republic of China, to DWY and GYP; and the Chinese Academy of Sciences |
| 通讯作者 | yangyahan@mail.kiz.ac.cn ; ypgeng@ynu.edu.cn |
| 中文摘要 | The Honghe-Hani landscape in China is a UNESCO World Natural Heritage site due to the beauty of its thousands of rice terraces, but these structures are in danger from the invasive crayfish Procambarus clarkii. Crayfish dig nest holes, which collapse terrace walls and destroy rice production. Under the current control strategy, farmers self-report crayfish and are issued pesticide, but this strategy is not expected to eradicate the crayfish nor to prevent their spread since farmers are not able to detect small numbers of crayfish. Thus, we tested whether environmental DNA (eDNA) from paddy-water samples could provide a sensitive detection method. In an aquarium experiment, Real-time Quantitative polymerase chain reaction (qPCR) successfully detected crayfish, even at a simulated density of one crayfish per average-sized paddy (with one false negative). In a field test, we tested eDNA and bottle traps against direct counts of crayfish. eDNA successfully detected crayfish in all 25 paddies where crayfish were observed and in none of the 7 paddies where crayfish were absent. Bottle-trapping was successful in only 68% of the crayfish-present paddies. eDNA concentrations also correlated positively with crayfish counts. In sum, these results suggest that single samples of eDNA are able to detect small crayfish populations, but not perfectly. Thus, we conclude that a program of repeated eDNA sampling is now feasible and likely reliable for measuring crayfish geographic range and for detecting new invasion fronts in the Honghe Hani landscape, which would inform regional control efforts and help to prevent the further spread of this invasive crayfish. |
| 收录类别 | SCI |
| 源URL | [http://159.226.149.26:8080/handle/152453/11585]  |
| 专题 | 昆明动物研究所_遗传资源与进化国家重点实验室 昆明动物研究所_动物生态学研究中心 昆明动物研究所_生命条形码中心 |
| 作者单位 | 1.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China 2.Kunming College of Life Science, University of the Chinese Academyof Sciences, Kunming, Yunnan, China 3.Institute of Ecology and Geobotany, School of Ecology and Environmental Science, Yunnan University, Kunming, Yunnan, China 4.Public Technical Service Center, Kunming Institute of Zoology, Chinese Academy of Science, Kunming, China 5.Kunming Biological Diversity Regional Center of Instruments, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China 6.School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, Norfolk, United Kingdom |
| 推荐引用方式 GB/T 7714 | Cai W,Ma ZX,Zhao GG,et al. Using eDNA to detect the distribution and density of invasive crayfish in the Honghe- Hani rice terrace World Heritage site[J]. PLos One,2017,15(5):e0177724. |
| APA | Cai W.,Ma ZX.,Zhao GG.,Geng YP[*].,Yu DW.,...&Wang WZ.(2017).Using eDNA to detect the distribution and density of invasive crayfish in the Honghe- Hani rice terrace World Heritage site.PLos One,15(5),e0177724. |
| MLA | Cai W,et al."Using eDNA to detect the distribution and density of invasive crayfish in the Honghe- Hani rice terrace World Heritage site".PLos One 15.5(2017):e0177724. |
入库方式： OAI收割
来源：昆明动物研究所
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