Rapid Functional and Sequence Differentiation of a Tandemly Repeated Species-Specific Multigene Family in Drosophila
文献类型：期刊论文
| 作者 | Clifton, Bryan D.1; Librado, Pablo2; Yeh, Shu-Dan3; Solares, Edwin S.1; Real, Daphne A.1; Jayasekera, Suvini U.1; Zhang, Wanting4; Shi, Mijuan4; Park, Ronni V.1; Magie, Robert D.1 |
| 刊名 | MOLECULAR BIOLOGY AND EVOLUTION
 |
| 出版日期 | 2017 |
| 卷号 | 34期号:1页码:51-65 |
| 关键词 | gene amplification functional diversification newly evolved gene Sdic Drosophila |
| ISSN号 | 0737-4038 |
| 英文摘要 | Gene clusters of recently duplicated genes are hotbeds for evolutionary change. However, our understanding of how mutational mechanisms and evolutionary forces shape the structural and functional evolution of these clusters is hindered by the high sequence identity among the copies, which typically results in their inaccurate representation in genome assemblies. The presumed testis-specific, chimeric gene Sdic originated, and tandemly expanded in Drosophila melanogaster, contributing to increased male-male competition. Using various types of massively parallel sequencing data, we studied the organization, sequence evolution, and functional attributes of the different Sdic copies. By leveraging long-read sequencing data, we uncovered both copy number and order differences from the currently accepted annotation for the Sdic region. Despite evidence for pervasive gene conversion affecting the Sdic copies, we also detected signatures of two episodes of diversifying selection, which have contributed to the evolution of a variety of C-termini and miRNA binding site compositions. Expression analyses involving RNA-seq datasets from 59 different biological conditions revealed distinctive expression breadths among the copies, with three copies being transcribed in females, opening the possibility to a sexually antagonistic effect. Phenotypic assays using Sdic knock-out strains indicated that should this antagonistic effect exist, it does not compromise female fertility. Our results strongly suggest that the genome consolidation of the Sdic gene cluster is more the result of a quick exploration of different paths of molecular tinkering by different copies than a mere dosage increase, which could be a recurrent evolutionary outcome in the presence of persistent sexual selection. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Evolutionary Biology ; Genetics & Heredity |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Evolutionary Biology ; Genetics & Heredity |
| 关键词[WOS] | DYNEIN INTERMEDIATE-CHAIN ; BASIC LOCAL ALIGNMENT ; SPERM-SPECIFIC GENE ; INSECTICIDE RESISTANCE ; NUCLEOTIDE-SEQUENCES ; DUPLICATED GENES ; MOLECULAR-BASIS ; X-CHROMOSOME ; EVOLUTION ; MELANOGASTER |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000396772000004 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/28955]  |
| 专题 | 水生生物研究所_水环境工程研究中心_期刊论文 |
| 作者单位 | 1.Univ Calif Irvine, Dept Ecol & Evolutionary Biol, Irvine, CA 92717 USA 2.Univ Copenhagen, Nat Hist Museum Denmark, Ctr GeoGenet, Copenhagen, Denmark 3.Natl Cent Univ, Dept Life Sci, Taoyuan, Zhongli Distric, Taiwan 4.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei Province, Peoples R China 5.Univ Essex, Sch Biol Sci, Colchester, Essex, England 6.Univ Barcelona, Dept Genet Microbiol & Estadist, Barcelona, Spain 7.Univ Barcelona, Inst Recerca Biodiversitat, Barcelona, Spain |
| 推荐引用方式 GB/T 7714 | Clifton, Bryan D.,Librado, Pablo,Yeh, Shu-Dan,et al. Rapid Functional and Sequence Differentiation of a Tandemly Repeated Species-Specific Multigene Family in Drosophila[J]. MOLECULAR BIOLOGY AND EVOLUTION,2017,34(1):51-65. |
| APA | Clifton, Bryan D..,Librado, Pablo.,Yeh, Shu-Dan.,Solares, Edwin S..,Real, Daphne A..,...&Ranz, Jose M..(2017).Rapid Functional and Sequence Differentiation of a Tandemly Repeated Species-Specific Multigene Family in Drosophila.MOLECULAR BIOLOGY AND EVOLUTION,34(1),51-65. |
| MLA | Clifton, Bryan D.,et al."Rapid Functional and Sequence Differentiation of a Tandemly Repeated Species-Specific Multigene Family in Drosophila".MOLECULAR BIOLOGY AND EVOLUTION 34.1(2017):51-65. |
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来源：水生生物研究所
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