Confinement Alters the Structure and Function of Calmodulin
文献类型：期刊论文
| 作者 | Xu, Guohua1; Cheng, Kai1,2; Wu, Qiong1; Liu, Maili1; Li, Conggang1 |
| 刊名 | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
 |
| 出版日期 | 2017-01-09 |
| 卷号 | 56期号:2页码:530-534 |
| 关键词 | calmodulin confinement NMR spectroscopy protein structures reverse micelles |
| 英文摘要 | Many cellular reactions involving proteins, including their biosynthesis, misfolding, and transport, occur in confined compartments. Despite its importance, a structural basis of understanding of how confined environments alter protein function is still lacking. Herein, we explore structure-function correlations of calmodulin (CaM), a multidomain protein involved in many calcium-mediated signaling pathways, in reverse micelles. Confinement dramatically alters CaM structure and function. The protein forms an extended structure in bulk water, but becomes compacted in reverse micelles. In addition, confinement changes the function of CaM. Specifically, the protein binds the MLCK, AcN19, and somatostatin peptides in dilute buffer, but binds only the MLCK and AcN19 peptides in reverse micelles. In summary, we determined a new CaM structure in reverse micelles and demonstrate that confinement can modulate both protein structure and function. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Multidisciplinary |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | MOLECULAR-STRUCTURE DETERMINATION ; REVERSE MICELLES ; XPLOR-NIH ; ENCAPSULATED PROTEINS ; BACKBONE DYNAMICS ; NMR-SPECTROSCOPY ; PARAMAGNETIC NMR ; LOW-TEMPERATURE ; UBIQUITIN ; SPECTRA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000394996100013 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/10027]  |
| 专题 | 武汉物理与数学研究所_磁共振基础研究部 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst,Collaborat Inn, State Key Lab Magnet Resonance & Atom & Mol Phys, Natl Ctr Magnet Resonance Wuhan,Wuhan Inst Phys &, Wuhan 430071, Peoples R China 2.Grad Univ Chinese Acad Sci, Beijing 100029, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Guohua,Cheng, Kai,Wu, Qiong,et al. Confinement Alters the Structure and Function of Calmodulin[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2017,56(2):530-534. |
| APA | Xu, Guohua,Cheng, Kai,Wu, Qiong,Liu, Maili,&Li, Conggang.(2017).Confinement Alters the Structure and Function of Calmodulin.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,56(2),530-534. |
| MLA | Xu, Guohua,et al."Confinement Alters the Structure and Function of Calmodulin".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 56.2(2017):530-534. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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