中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Transcriptome-Wide Identification and Prediction of miRNAs and Their Targets in Paris polyphylla var. yunnanensis by High-Throughput Sequencing Analysis

文献类型:期刊论文

作者Ling, Li-Zhen1,2; Zhang, Shu-Dong3; Zhao, Fan1; Yang, Jin-Long1; Song, Wen-Hui1; Guan, Shen-Min1; Li, Xin-Shu1; Huang, Zhuang-Jia1; Cheng, Le1,2,4
刊名INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
出版日期2017
卷号18期号:1
关键词Paris polyphylla var. yunnanensis miRNAs seed dormancy high-throughput sequencing technology
英文摘要Long dormancy period of seeds limits the large-scale artificial cultivation of the scarce Paris polyphylla var. yunnanensis, an important traditional Chinese medicine. Characterizing miRNAs and their targets is crucial to understanding the role of miRNAs during seed dormancy in this species. Considering the limited genome information of this species, we first sequenced and assembled the transcriptome data of dormant seeds and their seed coats as the reference genome. A total of 146,671 unigenes with an average length of 923 bp were identified and showed functional diversity based on different annotation methods. Two small RNA libraries from respective seeds and seed coats were sequenced and the combining data indicates that 263 conserved miRNAs belonging to at least 83 families and 768 novel miRNAs in 1174 transcripts were found. The annotations of the predicted putative targets of miRNAs suggest that these miRNAs were mainly involved in the cell, metabolism and genetic information processing by direct and indirect regulation patterns in dormant seeds of P. polyphylla var. yunnanensis. Therefore, we provide the first known miRNA profiles and their targets, which will assist with further study of the molecular mechanism of seed dormancy in P. polyphylla var. yunnanensis.
类目[WOS]Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]SEED-GERMINATION ; MICRORNAS ; GENES ; THERMOINHIBITION ; STRATIFICATION ; BIOSYNTHESIS ; EXPRESSION ; GLYCOSIDES ; BIOLOGY ; PLANTS
收录类别SCI
语种英语
WOS记录号WOS:000393030600213
源URL[http://ir.kib.ac.cn/handle/151853/51009]  
专题昆明植物研究所_中国西南野生生物种质资源库
作者单位1.BGI Yunnan, Kunming 650106, Peoples R China
2.BGI Shenzhen, China Natl GeneBank, Shenzhen 518083, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming 650201, Peoples R China
4.Dali Univ, Coll Clin Med, Coll Basic Med Sci, Dali 671000, Peoples R China
推荐引用方式
GB/T 7714
Ling, Li-Zhen,Zhang, Shu-Dong,Zhao, Fan,et al. Transcriptome-Wide Identification and Prediction of miRNAs and Their Targets in Paris polyphylla var. yunnanensis by High-Throughput Sequencing Analysis[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2017,18(1).
APA Ling, Li-Zhen.,Zhang, Shu-Dong.,Zhao, Fan.,Yang, Jin-Long.,Song, Wen-Hui.,...&Cheng, Le.(2017).Transcriptome-Wide Identification and Prediction of miRNAs and Their Targets in Paris polyphylla var. yunnanensis by High-Throughput Sequencing Analysis.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,18(1).
MLA Ling, Li-Zhen,et al."Transcriptome-Wide Identification and Prediction of miRNAs and Their Targets in Paris polyphylla var. yunnanensis by High-Throughput Sequencing Analysis".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 18.1(2017).

入库方式: OAI收割

来源:昆明植物研究所

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