Regulation of enzyme activity and stability through positional interaction with polyurethane nanofibers.
文献类型：期刊论文
| 作者 | Ji, Xiaoyuan1,2; Su, Zhiguo1; Liu, Chunxia1; Wang, Ping1,3,4; Zhang, Songping1 |
| 刊名 | BIOCHEMICAL ENGINEERING JOURNAL
 |
| 出版日期 | 2017-05-15 |
| 卷号 | 121页码:147-155 |
| 关键词 | Nano-scale Microenvironment Hollow nanofiber Co-axial electrospinning Confinement effect Transesterification |
| ISSN号 | 1369-703X |
| 英文摘要 | Nanofibrous membranes have been adopted as enzyme carriers via surface adsorption, covalent cross linking, and direct electrospinning-embedment showing great advantages and successes over other nanostructured materials, and especially the newly developed hollow nanofibers were proven as an ideal scaffold for through in-situ encapsulation of multiple enzymes during co-axial electrospinning. Here, the coaxial electrospinning-encapsulation method based on hollow nanofiber structures, together with three previous strategies, which are all based on solid-structured nanofibers, were adopted to immobilize a-chymotrypsin (CT). The feasibilities and advantages of hollow nanofibers encapsulated enzyme are fully demonstrated by the catalytic kinetics of the immobilized CT for hydrolysis and transesterification. Compared to a solid nanofibers-based enzyme, the hollow nanofibers encapsulated CT show the highest catalytic efficiency for both hydrolysis and transesterification. Besides, the confinement effect provided by the nano-scaled hollow chamber not only facilitated molecular interactions between enzymes and substrates, but also enhanced the enzymes stability largely. (C) 2017 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Engineering |
| 关键词[WOS] | HOLLOW NANOFIBERS ; POTENTIAL APPLICATIONS ; POLYMER NANOFIBERS ; IMMOBILIZATION ; PERFORMANCE ; BIOSENSORS ; SUPPORTS ; STABILIZATION ; BIOCATALYSIS ; MEMBRANES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000398752600016 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/22457]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA 4.Univ Minnesota, Biotechnol Inst, St Paul, MN 55108 USA |
| 推荐引用方式 GB/T 7714 | Ji, Xiaoyuan,Su, Zhiguo,Liu, Chunxia,et al. Regulation of enzyme activity and stability through positional interaction with polyurethane nanofibers.[J]. BIOCHEMICAL ENGINEERING JOURNAL,2017,121:147-155. |
| APA | Ji, Xiaoyuan,Su, Zhiguo,Liu, Chunxia,Wang, Ping,&Zhang, Songping.(2017).Regulation of enzyme activity and stability through positional interaction with polyurethane nanofibers..BIOCHEMICAL ENGINEERING JOURNAL,121,147-155. |
| MLA | Ji, Xiaoyuan,et al."Regulation of enzyme activity and stability through positional interaction with polyurethane nanofibers.".BIOCHEMICAL ENGINEERING JOURNAL 121(2017):147-155. |
入库方式： OAI收割
来源：过程工程研究所
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