基于上转换发光技术的生物传感器及其应用
文献类型：期刊论文
| ; | |
| 作者 | 赵永凯 ; 周蕾 ; 黄惠杰 ; 杨瑞馥 ; 黄立华 ; 任冰强 ; 卢健 ; 王向朝 |
| 刊名 | 光学学报
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| 出版日期 | 2005 |
| 卷号 | 25期号:6页码:841 |
| 关键词 | 光学计量 optical metrology 传感器技术 sensor technology 生物传感器 biosensor 上转换发光技术 up-converting phosphor technology 上转换发光材料 up-converting phosphor material |
| ISSN号 | 0253-2239 |
| 其他题名 | Up-converting phosphor technology-based biosensor and its application |
| 中文摘要 | 为实现对特定生物分子的高灵敏度快速检测与分析。采用上转换发光材料作为标记物，研制成功一台基于上转换发光技术的新型光学免疫生物传感器。该传感器利用上转换发光材料在红外光激发下发射可见磷光的特性，通过对免疫层析试纸条上经生物反应而结合上去的上转换发光材料颗粒的含量进行检测，计算出被测样品中特定生物分子的浓度。实验结果表明，该传感器具有较好的生物特异性，对兔抗鼠疫免疫球蛋白(IgG)标准样品的检测灵敏度达到ng／ml量级，并在200～6000ng／ml浓度范围内具有良好的线性响应特性，相关系数R^2≥0．95；; An up-converting phosphor technology-based biosensor (UPT-based biosensor) was developed for immunoassay and fast detecting using up-converting phosphor (UCP) as the biological marker. UCP can emit visible light when excited by infrared light. Through detecting and analyzing the content of UCP particle on the test strip after immunoreaction, the UPT-based biosensor can obtain the concentration information of target analyte in the sample. The UPT system realized the quantitative detection and had good ability to meet the need of some emergencies. High sensitivity (nanogram/ml), good linear relationship in the concentration range of 200～6000 ng/ml and an excellent correlation (R2≥0.95) were verified by quantitative detection results. The sensitivity of the UPT-based biosensor was better than that of the indirect hemagglutination test in the practical application. All the results were comparable with that obtained by Western Blot detection. The UPT-based biosensor had stable, reliable and sensitive working performances, and it can meet the need of some various applications such as rapid immunoassay, chemical and biological detection and so on. |
| 分类号 | TP212.3 |
| 收录类别 | ei |
| 语种 | 中文 |
| 公开日期 | 2009-09-24 ; 2010-10-12 |
| 源URL | [http://ir.siom.ac.cn/handle/181231/6225]  |
| 专题 | 上海光学精密机械研究所_精密光电测控研究与发展中心 |
| 推荐引用方式 GB/T 7714 | 赵永凯,周蕾,黄惠杰,等. 基于上转换发光技术的生物传感器及其应用, Up-converting phosphor technology-based biosensor and its application[J]. 光学学报,2005,25(6):841, 847. |
| APA | 赵永凯.,周蕾.,黄惠杰.,杨瑞馥.,黄立华.,...&王向朝.(2005).基于上转换发光技术的生物传感器及其应用.光学学报,25(6),841. |
| MLA | 赵永凯,et al."基于上转换发光技术的生物传感器及其应用".光学学报 25.6(2005):841. |
入库方式： OAI收割
来源：上海光学精密机械研究所
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