Direct detection of Yersinia pestis from the infected animal specimens by a fiber optic biosensor
文献类型：期刊论文
| 作者 | Wei Hua ; Zhao Yongkai ; Bi Yujing ; Liu Haihong ; Guo Zhaobiao ; Song Yajun ; Zhai Junhui ; Huang HJ(黄惠杰) ; Yang Ruifu |
| 刊名 | sens. actuator b-chem.
 |
| 出版日期 | 2007 |
| 卷号 | 123期号:1页码:204 |
| 关键词 | fiber optic biosensor sandwich immunoassay Y. pestis |
| ISSN号 | 0925-4005 |
| 中文摘要 | the fob-3, anew type fiber optic biosensor, is designed to rapidly detect a variety of biological agents or analytes with better stability, sensitivity and specificity. in order to detect y. pestis, a sandwich immunoassay was developed by using the purified antibody against antigen fi immobilized on polystyrene probes as the capture antibody and the monoclonal antibody-cy5 conjugate as the detector. after a series of optimization for the stability, sensitivity and specificity of the fob-3, 50-1000 ng/ml of antigen fi and 6 x 10(1)-6 x 10(7) cfu/ml y. pestis could be detected constantly in about 20 min, and y pestis could be detected specifically from y. pseudotuberculosis, y. enterocolitica, b. anthracis and e. coli. then, 39 blind samples, including 27 tissues of mice infected with y pestis and 12 tissues of healthy mice as negative control, were detected with the fob-3. 92.6% infected tissues were identified from the tissues of healthy mice and the tissues containing more than 100 cfu/ml bacteria could be detected by the biosensor. the results demonstrated the feasibility of the fob-3 as an effective method to detect y. pestis rapidly and directly from the infected animal specimens with the advantage of portability, simple-operation as well as high sensitivity and specificity. (c) 2006 elsevier b.v. all rights reserved. |
| 收录类别 | EI |
| 语种 | 英语 |
| WOS记录号 | WOS:000246171200031 |
| 公开日期 | 2009-09-24 |
| 源URL | [http://ir.siom.ac.cn/handle/181231/6263]  |
| 专题 | 上海光学精密机械研究所_精密光电测控研究与发展中心 |
| 推荐引用方式 GB/T 7714 | Wei Hua,Zhao Yongkai,Bi Yujing,et al. Direct detection of Yersinia pestis from the infected animal specimens by a fiber optic biosensor[J]. sens. actuator b-chem.,2007,123(1):204, 210. |
| APA | Wei Hua.,Zhao Yongkai.,Bi Yujing.,Liu Haihong.,Guo Zhaobiao.,...&Yang Ruifu.(2007).Direct detection of Yersinia pestis from the infected animal specimens by a fiber optic biosensor.sens. actuator b-chem.,123(1),204. |
| MLA | Wei Hua,et al."Direct detection of Yersinia pestis from the infected animal specimens by a fiber optic biosensor".sens. actuator b-chem. 123.1(2007):204. |
入库方式： OAI收割
来源：上海光学精密机械研究所
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