Effects of forced solution flow on lysozyme crystal growth
文献类型：期刊论文
| 作者 | Yu Y(于泳) ; Liu YH(刘玉红); Wang X(王翾); Oberthür D; Dierks K; Betzel C
|
| 刊名 | Crystal Research and Technology
 |
| 出版日期 | 2010 |
| 卷号 | 45期号:4页码:380-386 |
| 通讯作者邮箱 | yuyong@imech.ac.cn |
| 关键词 | protein crystallization, fluid flow, impurities, mass transfer, lysozyme. |
| ISSN号 | 0232-1300 |
| 通讯作者 | Yong Yu(于泳） |
| 合作状况 | 国际 |
| 中文摘要 | In order to study quantitatively the effects of forced solution on crystal growth, we designed a new set of experimental equipment, in particular, a microchannel mixer was used as crystallization container so that the consumption of protein samples was much reduced and thus an exact syringe pump could be used for precise control of the flow rates. Since the mixer’s section was designed to be rectangular, the solution velocity in its center was steady and constant, and thus repeatable experiments were facilitated. Experimental results showed that the effects of forced solution on protein crystal growth were different under different levels of supersaturation, and new results were obtained for cases of high supersaturation. When the supersaturation is σ = 2.3, with increasing flow rates the growth rates of the lysozyme crystal’s (110) face hardly change when the flow rates are lower than 1300 μm/s, and decrease quickly afterwards. When the flow rate reaches 2000 μm/s, the crystal nearly ceases to grow. When the supersaturation is σ = 2.7, with increasing flow rates the (110) face growth rates increase at the beginning then reach the maximum values at 1700 μm/s – 1900 μm/s and decrease afterwards, approaching zero or so when the flow rate reaches 12000 μm/s. The higher the supersaturation, the larger the flow rate at which the crystal ceases to grow. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim |
| 学科主题 | 交叉与边缘领域的力学 |
| 收录类别 | SCI ; EI |
| 原文出处 | http://onlinelibrary.wiley.com/doi/10.1002/crat.200900721/abstract |
| 语种 | 英语 |
| WOS记录号 | WOS:000276599200007 |
| 公开日期 | 2010-12-08 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/43244]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Yu Y,Liu YH,Wang X,et al. Effects of forced solution flow on lysozyme crystal growth[J]. Crystal Research and Technology,2010,45(4):380-386. |
| APA | Yu Y,Liu YH,Wang X,Oberthür D,Dierks K,&Betzel C.(2010).Effects of forced solution flow on lysozyme crystal growth.Crystal Research and Technology,45(4),380-386. |
| MLA | Yu Y,et al."Effects of forced solution flow on lysozyme crystal growth".Crystal Research and Technology 45.4(2010):380-386. |
入库方式： OAI收割
来源：力学研究所
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