An ATP-dependent mechanism mediates intercellular calcium signaling in bone cell network under single cell nanoindentation
文献类型：期刊论文
| 作者 | Huo B(霍波) ; Lu XL; Costa KD; Xu QB; Guo XE
|
| 刊名 | Cell Calcium
 |
| 出版日期 | 2010 |
| 卷号 | 47期号:3页码:234-241 |
| 通讯作者邮箱 | ed.guo@columbia.edu |
| ISSN号 | 0143-4160 |
| 通讯作者 | Guo XE |
| 合作状况 | 国际 |
| 中文摘要 | To investigate the roles of intercellular gap junctions and extracellular ATP diffusion in bone cell calcium signaling propagation in bone tissue, in vitro bone cell networks were constructed by using microcontact printing and self-assembled monolayer technologies. In the network, neighboring cells were interconnected through functional gap junctions. A single cell at the center of the network was mechanically stimulated by using an AFM nanoindenter. Intracellular calcium ([Ca2+](i)) responses of the bone cell network were recorded and analyzed. In the untreated groups, calcium propagation from the stimulated cell to neighboring cells was observed in 40% of the tests. No significant difference was observed in this percentage when the intercellular gap junctions were blocked. This number, however, decreased to 10% in the extracellular ATP-pathway-blocked group. When both the gap junction and ATP pathways were blocked, intercellular calcium waves were abolished. When the intracellular calcium store in ER was depleted, the indented cell can generate calcium transients, but no [Ca2+](i) signal can be propagated to the neighboring cells. No [Ca2+](i) response was detected in the cell network when the extracellular calcium source was removed. These findings identified the biochemical pathways involved in the calcium signaling propagation in bone cell networks. Published by Elsevier Ltd. |
| 类目[WOS] | Cell Biology |
| 研究领域[WOS] | Cell Biology |
| 关键词[WOS] | OSTEOBLASTIC CELLS ; INOSITOL TRISPHOSPHATE ; RECEPTOR ACTIVATION ; EXTRACELLULAR ATP ; EPITHELIAL-CELLS ; GAP-JUNCTIONS ; CA2+ SIGNALS ; PROPAGATION ; WAVES ; COMMUNICATION |
| 收录类别 | SCI |
| 资助信息 | NIH [R21 AR052417] |
| 原文出处 | http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WCC-4Y41MMD-1&_user=1999820&_coverDate=03%2F31%2F2010&_rdoc=1&_fmt=high&_orig=gateway&_origin=gateway&_sort=d&_docanchor=&view=c&_searchStrId=1695614825&_rerunOrigin=google&_acct=C000050602&_version=1&_urlVersion=0&_userid=1999820&md5=5f8fe7a447bbc30f5eaa2ddc182d9028&searchtype=a |
| 语种 | 英语 |
| WOS记录号 | WOS:000276430100004 |
| 公开日期 | 2011-03-01 ; 2011-03-30 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/43669]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Huo B,Lu XL,Costa KD,et al. An ATP-dependent mechanism mediates intercellular calcium signaling in bone cell network under single cell nanoindentation[J]. Cell Calcium,2010,47(3):234-241. |
| APA | 霍波,Lu XL,Costa KD,Xu QB,&Guo XE.(2010).An ATP-dependent mechanism mediates intercellular calcium signaling in bone cell network under single cell nanoindentation.Cell Calcium,47(3),234-241. |
| MLA | 霍波,et al."An ATP-dependent mechanism mediates intercellular calcium signaling in bone cell network under single cell nanoindentation".Cell Calcium 47.3(2010):234-241. |
入库方式： OAI收割
来源：力学研究所
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