Intercellular calcium wave propagation in linear and circuit-like bone cell networks
文献类型：期刊论文
| 作者 | Huo B(霍波) ; Lu XL; Guo XE; Huo B(霍波)
|
| 刊名 | Philosophical Transactions of The Royal Society A-Mathematical Physical and Engineering Sciences
 |
| 出版日期 | 2010 |
| 卷号 | 368期号:1912页码:617-633 |
| 通讯作者邮箱 | ed.guo@columbia.edu |
| ISSN号 | 1364-503X |
| 通讯作者 | Guo XE |
| 合作状况 | 国际 |
| 中文摘要 | In the present study, the mechanism of intercellular calcium wave propagation in bone cell networks was identified. By using micro-contact printing and self-assembled monolayer technologies, two types of in vitro bone cell networks were constructed: open-ended linear chains and looped hexagonal networks with precisely controlled intercellular distances. Intracellular calcium responses of the cells were recorded and analysed when a single cell in the network was mechanically stimulated by nano-indentation. The looped cell network was shown to be more efficient than the linear pattern in transferring calcium signals from cell to cell. This phenomenon was further examined by pathway-inhibition studies. Intercellular calcium wave propagation was significantly impeded when extracellular adenosine triphosphate (ATP) in the medium was hydrolysed. Chemical uncoupling of gap junctions, however, did not significantly decrease the transferred distance of the calcium wave in the cell networks. Thus, it is extracellular ATP diffusion, rather than molecular transport through gap junctions, that dominantly mediates the transmission of mechanically elicited intercellular calcium waves in bone cells. The inhibition studies also demonstrated that the mechanical stimulation-induced calcium responses required extracellular calcium influx, whereas the ATP-elicited calcium wave relied on calcium release from the calcium store of the endoplasmic reticulum. |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | FLUID-FLOW ; OSTEOBLASTIC CELLS ; GAP-JUNCTIONS ; ATP ; MECHANOTRANSDUCTION ; COMMUNICATION ; OSCILLATIONS ; PERIODS ; ACID |
| 收录类别 | SCI |
| 资助信息 | NIH [R21 AR052417] |
| 原文出处 | http://dx.doi.org/10.1098/rsta.2009.0221 |
| 语种 | 英语 |
| WOS记录号 | WOS:000273233400006 |
| 公开日期 | 2011-03-01 ; 2011-03-30 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/43687]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Huo B,Lu XL,Guo XE,et al. Intercellular calcium wave propagation in linear and circuit-like bone cell networks[J]. Philosophical Transactions of The Royal Society A-Mathematical Physical and Engineering Sciences,2010,368(1912):617-633. |
| APA | 霍波,Lu XL,Guo XE,&Huo B.(2010).Intercellular calcium wave propagation in linear and circuit-like bone cell networks.Philosophical Transactions of The Royal Society A-Mathematical Physical and Engineering Sciences,368(1912),617-633. |
| MLA | 霍波,et al."Intercellular calcium wave propagation in linear and circuit-like bone cell networks".Philosophical Transactions of The Royal Society A-Mathematical Physical and Engineering Sciences 368.1912(2010):617-633. |
入库方式： OAI收割
来源：力学研究所
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