Feasibility of Protein a for the Oriented Immobilization of Immunoglobulin on Silicon Surface for a Biosensor with Imaging Ellipsometry
文献类型：期刊论文
| 作者 | Wang ZH(王战会); Jin G(靳刚)
|
| 刊名 | Journal of Biochemical and Biophysical Methods
 |
| 出版日期 | 2003 |
| 卷号 | 57期号:3页码:203-211 |
| ISSN号 | 0165-022X |
| 通讯作者 | Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, 15 Bei Si Huan W Rd, Beijing 100080, Peoples R China. |
| 中文摘要 | The feasibility of using protein A to immobilize antibody on silicon surface for a biosensor with imaging ellipsometry was presented in this study. The amount of human IgG bound with anti-IgG immobilized by the protein A on silicon surface was much more than that bound with anti-IgG immobilized by physical adsorption. The result indicated that the protein A could be used to immobilize antibody molecules in a highly oriented manner and maintain antibody molecular functional configuration on the silicon surface. High reproducibility of the amount of antibody immobilization and homogenous antibody adsorption layer on surfaces could be obtained by this immobilization method. Imaging ellipsometry has been proven to be a fast and reliable detection method and sensitive enough to detect small changes in a molecular monolayer level. The combination of imaging ellipsometry and surface modification with protein A has the potential to be further developed into an efficient immunoassay protein chip. |
| 学科主题 | 力学 |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | VISUALIZATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000186079000003 |
| 公开日期 | 2007-06-15 ; 2007-12-05 ; 2009-06-23 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/16687]  |
| 专题 | 力学研究所_力学所知识产出(1956-2008) |
| 推荐引用方式 GB/T 7714 | Wang ZH,Jin G. Feasibility of Protein a for the Oriented Immobilization of Immunoglobulin on Silicon Surface for a Biosensor with Imaging Ellipsometry[J]. Journal of Biochemical and Biophysical Methods,2003,57(3):203-211. |
| APA | 王战会,&靳刚.(2003).Feasibility of Protein a for the Oriented Immobilization of Immunoglobulin on Silicon Surface for a Biosensor with Imaging Ellipsometry.Journal of Biochemical and Biophysical Methods,57(3),203-211. |
| MLA | 王战会,et al."Feasibility of Protein a for the Oriented Immobilization of Immunoglobulin on Silicon Surface for a Biosensor with Imaging Ellipsometry".Journal of Biochemical and Biophysical Methods 57.3(2003):203-211. |
入库方式： OAI收割
来源：力学研究所
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