Determination of SARS-coronavirus by a microfluidic chip system
文献类型：期刊论文
| 作者 | Zhou, ZM; Liu, DY; Zhong, RT; Dai, ZP; Wu, DP; Wang, H; Du, YG; Xia, ZN; Zhang, LP; Mei, XD |
| 刊名 | electrophoresis
 |
| 出版日期 | 2004-09-01 |
| 卷号 | 25期号:17页码:3032-3039 |
| 关键词 | microfluidic chip system miniaturization multiplex reverse transcription-polymerase chain reaction on-line chip polymerase chain reaction severe acute respiratory syndrome |
| 产权排序 | 1;1 |
| 英文摘要 | we have developed a new experimental system based on a microfluidic chip to determine severe acute respiratory syndrome coronavirus (sars-cov). the system includes a laser-induced fluorescence microfluidic chip analyzer, a glass microchip for both polymerase chain reaction (pcr) and capillary electrophoresis, a chip thermal cycler based on dual peltier thermoelectric elements, a reverse transcription-polymerase chain reaction (rt-pcr) sars diagnostic kit, and a dna electrophoretic sizing kit. the system allows efficient cdna amplification of sars-cov followed by electrophoretic sizing and detection on the same chip. to enhance the reliability of rt-pcr on sars-cov detection, duplex pcr was developed on the microchip. the assay was carried out on a home-made microfluidic chip system. the positive and the negative control were cdna fragments of sars-cov and parainfluenza virus, respectively. the test results showed that 17 positive samples were obtained among 18 samples of nasopharyngeal swabs from clinically diagnosed sars patients. however, 12 positive results from the same 18 samples were obtained by the conventional rt-pcr with agarose gel electrophoresis detection. the sars virus species can be analyzed with high positive rate and rapidity on the microfluidic chip system. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | clinical-application ; microchip pcr ; rapid pcr ; dna ; electrophoresis ; instrument ; assay |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000223938700019 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/81099]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Harvard Univ, Sch Med, Dana Fabre Canc Res Inst, Boston, MA 02115 USA 3.Wyeth Pharmaceut Inc, Genom Dept, Cambridge, MA USA |
| 推荐引用方式 GB/T 7714 | Zhou, ZM,Liu, DY,Zhong, RT,et al. Determination of SARS-coronavirus by a microfluidic chip system[J]. electrophoresis,2004,25(17):3032-3039. |
| APA | Zhou, ZM.,Liu, DY.,Zhong, RT.,Dai, ZP.,Wu, DP.,...&Lin, BC.(2004).Determination of SARS-coronavirus by a microfluidic chip system.electrophoresis,25(17),3032-3039. |
| MLA | Zhou, ZM,et al."Determination of SARS-coronavirus by a microfluidic chip system".electrophoresis 25.17(2004):3032-3039. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。