Determination of organophosphate and carbamate pesticides based on enzyme inhibition using a pH-sensitive fluorescence probe
文献类型：期刊论文
| 作者 | Jin, SY; Xu, ZC; Chen, JP; Liang, XM; Wu, YN; Qian, XH |
| 刊名 | analytica chimica acta
 |
| 出版日期 | 2004-10-04 |
| 卷号 | 523期号:1页码:117-123 |
| 关键词 | acetylcholinesterase fluorescence probe flow-injection analysis organophosphate pesticides carbamate pesticides vegetable juices |
| 产权排序 | 1;1 |
| 英文摘要 | a flow injection system for the determination of organophosphate and carbamate pesticides is described. a sensitive fluorescence probe was synthesized and used as the ph indicator to detect the inhibition of the enzyme acetylcholinesterase (acne). the percentage inhibition of enzyme activity is correlated to the pesticide concentration. several parameters influencing the performance of the system are discussed. the detection limits of 3.5, 50, 12 and 25 mug/l for carbofuran, carbaryl, paraoxon and dichlorvos, in pure water, respectively were achieved with an incubation time of 10 min. a complete cycle of analysis, including incubation time, took 14 min. the detection system has been applied to the determination of carbofuran in spiked vegetable juices (chinese cabbage and cole), achieving recovery values between 93.2 and 107% for chinese cabbage juice and 108 and 118% for cole juice at the different concentration levels assayed. (c) 2004 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | flow-injection analysis ; immobilized acetylcholinesterase ; biosensor ; paraoxon ; cholinesterase ; sensor ; insecticides ; emission ; reactor ; samples |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000224039700017 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/81215]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116012, Peoples R China 2.Dalian Univ Technol, Dept Fine Chem Engn, Dalian 116012, Peoples R China 3.Natl Inst Nutr & Food Safety, Chinese Ctr Dis Control & Prevent, Beijing 100050, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jin, SY,Xu, ZC,Chen, JP,et al. Determination of organophosphate and carbamate pesticides based on enzyme inhibition using a pH-sensitive fluorescence probe[J]. analytica chimica acta,2004,523(1):117-123. |
| APA | Jin, SY,Xu, ZC,Chen, JP,Liang, XM,Wu, YN,&Qian, XH.(2004).Determination of organophosphate and carbamate pesticides based on enzyme inhibition using a pH-sensitive fluorescence probe.analytica chimica acta,523(1),117-123. |
| MLA | Jin, SY,et al."Determination of organophosphate and carbamate pesticides based on enzyme inhibition using a pH-sensitive fluorescence probe".analytica chimica acta 523.1(2004):117-123. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。