Determination and identification of isoflavonoids in Radix astragali by matrix solid-phase dispersion extraction and high-performance liquid chromatography with photodiode array and mass spectrometric detection
文献类型：期刊论文
| 作者 | Xiao, HB; Krucker, M; Albert, K; Liang, XM |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2004-04-02 |
| 卷号 | 1032期号:1-2页码:117-124 |
| 关键词 | Radix astragali matrix solid-phase dispersion extraction methods plant materials Astragalus spp. isoflavonoids flavonoids |
| 产权排序 | 2;4 |
| 英文摘要 | the isoflavonoids in radix astragali were determined and identified by hplc-photodiode array detection-ms after extraction employing matrix solid-phase dispersion (mspd). as a new sample preparation method for r. astragali, the mspd procedure was optimized, validated and compared with conventional methods including ultrasonic and soxhlet extraction. the amounts of two major components in this herb, formononetin (6) and ononin (2), were determined based on their authentic standards. four major isoflavonoids, formononetin (6), ononin (2), calycosin (5) and its glycoside (1), and three minor isoflavonoids, (6ar,11ar)-3-hydroxy-9, 10-dimethoxypterocarpan (7), its glycoside (3), and (3r)-7,2'-dihydroxy-3',4'-dimethoxyisoflavone-7-o-beta-d-glycoside (4), were identified based on their characteristic two-band uv spectra and [m + h](+), [aglycone + h](+) and [a1 + h](+) ions, etc. the combined mspd and hplc-dad-ms method was suitable for quantitative and qualitative determination of the isoflavonoids in r. astragali. (c) 2003 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | magnetic-resonance-spectroscopy ; natural-products ; combination ; flavonoids |
| 收录类别 | ISTP ; SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000220121200018 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/81493]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Univ Tubingen, Inst Organ Chem, D-72076 Tubingen, Germany 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiao, HB,Krucker, M,Albert, K,et al. Determination and identification of isoflavonoids in Radix astragali by matrix solid-phase dispersion extraction and high-performance liquid chromatography with photodiode array and mass spectrometric detection[J]. journal of chromatography a,2004,1032(1-2):117-124. |
| APA | Xiao, HB,Krucker, M,Albert, K,&Liang, XM.(2004).Determination and identification of isoflavonoids in Radix astragali by matrix solid-phase dispersion extraction and high-performance liquid chromatography with photodiode array and mass spectrometric detection.journal of chromatography a,1032(1-2),117-124. |
| MLA | Xiao, HB,et al."Determination and identification of isoflavonoids in Radix astragali by matrix solid-phase dispersion extraction and high-performance liquid chromatography with photodiode array and mass spectrometric detection".journal of chromatography a 1032.1-2(2004):117-124. |
入库方式： OAI收割
来源：大连化学物理研究所
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