Detection of K-ras exon 1 mutations by constant denaturant capillary electrophoresis
文献类型：期刊论文
| 作者 | Zhao, CX; Xu, GW; Shi, XZ; Ma, JM; Lu, S; Yang, Q |
| 刊名 | biomedical chromatography
 |
| 出版日期 | 2004-10-01 |
| 卷号 | 18期号:8页码:538-541 |
| 关键词 | constant denaturant capillary electrophoresis K-ras gene mutation detection |
| 产权排序 | 1;1 |
| 英文摘要 | among various mutation detection methods, constant denaturant capillary electrophoresis (cdce) is one of the most common techniques for rapid identification of known or unknown mutations. in this report, a cdce analysis method with homemade linear polyacrylamide (lpa) kit was developed on abi 310 genetic analyzer, the effect and relationship of various denaturing factors in cdce analysis were investigated and k-ras gene mutations of 31 coloerctal cancer patients were detected. results indicate that, with the increase of chemical danaturant concentration, the optimum temperature was lowered, and when the concentration of urea (formamide) was higher than 7 m (40%), the homoduplex and heteroduplex of mutant samples were separated with difficulty. detection results of k-ras gene in colorectal samples indicated that mutations were present in eight (26%) of 31 patients; most mutations were localized in codon 12, which is thought to be a critical step and plays an important role in human colorectal carcinogenesisas. copyright (c) 2004 john wiley sons, ltd. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; biochemistry & molecular biology ; chemistry, analytical ; pharmacology & pharmacy |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry ; pharmacology & pharmacy |
| 关键词[WOS] | gene ; dna ; cdce ; separation ; p53 |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000224314500008 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/81583]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China 2.Dalian Med Univ, Secondary Affiliated Hosp, Dalian 116027, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, CX,Xu, GW,Shi, XZ,et al. Detection of K-ras exon 1 mutations by constant denaturant capillary electrophoresis[J]. biomedical chromatography,2004,18(8):538-541. |
| APA | Zhao, CX,Xu, GW,Shi, XZ,Ma, JM,Lu, S,&Yang, Q.(2004).Detection of K-ras exon 1 mutations by constant denaturant capillary electrophoresis.biomedical chromatography,18(8),538-541. |
| MLA | Zhao, CX,et al."Detection of K-ras exon 1 mutations by constant denaturant capillary electrophoresis".biomedical chromatography 18.8(2004):538-541. |
入库方式： OAI收割
来源：大连化学物理研究所
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