Applications of homemade kit in mutation detection of genes
文献类型：期刊论文
| 作者 | Zhao, CX; Xu, GW; Shi, XZ; Ma, JM; Zhang, Y; Lu, S; Yang, Q |
| 刊名 | science in china series b-chemistry
 |
| 出版日期 | 2004-04-01 |
| 卷号 | 47期号:2页码:166-172 |
| 关键词 | single nucleotide polymorphism (SNP) single-strand conformation pollymorphism (SSCP) heteroduplex analysis (HA) SNaPshot linear polyacrylamide (LPA) polydimethylacrylamide (PDMA) |
| 产权排序 | 1;1 |
| 英文摘要 | several methods of mutation detection, such as single-strand conformation polymorphism (sscp), tandem sscp/heteroduplex analysis and snapshot analysis were developed using homemade kit on abi 310 genetic analyzer, and were successfully applied to mutation detection of 31 colorectal tumor samples. the sieving capability of homemade kit and commercial kit were compared, results demonstrate that homemade kit has higher resolution and shorter analysis time. in clinical tumor samples, 26% k-ras (exon 1) and 24% p53 (exons 7-8) were found to have mutations, and all mutations were single point variations. a majority of mutations occurred in one gene, only 1 tumor contained alterations in the two genes, which indicates that development of colorectal cancer lies on alternate pathways, and may correlate with different gene mutations. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | strand conformation polymorphism ; single nucleotide polymorphisms ; capillary-electrophoresis ; gel-electrophoresis ; dna separation ; resolution ; polymers |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000221119100012 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/81587]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China 2.Dalian Med Univ, Affiliated Hosp 2, Cell Lab, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, CX,Xu, GW,Shi, XZ,et al. Applications of homemade kit in mutation detection of genes[J]. science in china series b-chemistry,2004,47(2):166-172. |
| APA | Zhao, CX.,Xu, GW.,Shi, XZ.,Ma, JM.,Zhang, Y.,...&Yang, Q.(2004).Applications of homemade kit in mutation detection of genes.science in china series b-chemistry,47(2),166-172. |
| MLA | Zhao, CX,et al."Applications of homemade kit in mutation detection of genes".science in china series b-chemistry 47.2(2004):166-172. |
入库方式： OAI收割
来源：大连化学物理研究所
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