Determination of urinary oxidative DNA damage marker 8-hydroxy-2 '-deoxyguanosine and the association with cigarette smoking
文献类型：期刊论文
| 作者 | Yao, QH; Mei, SR; Weng, QF; Zhang, PD; Yang, Q; Wu, CY; Xu, GW |
| 刊名 | talanta
 |
| 出版日期 | 2004-06-17 |
| 卷号 | 63期号:3页码:617-623 |
| 关键词 | 8-hydroxy-2 '-deoxyguanosine urine cigarette smoking capillary electrophoresis end-column amperometric detection |
| 产权排序 | 1;1 |
| 英文摘要 | 8-hydroxy-2'-deoxyguanosine (8ohdg) has been widely used as a biomarker of oxidative dna damage in both animal models and human studies. to evaluate the effect of cigarette smoking on oxidative stress, we studied the levels of urinary 8ohdg from smokers and non-smokers and investigated the association with cigarette smoking. the urinary 8ohdg concentrations were determinated by capillary electrophoresis with end-column amprometric detection (ce-ad) after a single-step solid phase extraction (spe), and then quantitatively expressed as a function of creatinine excretion. to increase the concentration sensitivity, a dynamic ph junction was used and the focusing effect was obvious when using 30 mm phosphate (ph 6.50) as sample matrix. the limit of detection is 4.3 nm (signal-to-noise ratio s/n = 3). the relative standard deviation (r.s.d.) was 1.1% for peak current, and 2.3% for migration time. based on the selected ce-ad method, it was found that the mean value of urinary 8ohdg levels in the smokers significantly higher than that in non-smokers (31.4 +/- 18.9 nm versus 14.4 +/- 7.6 nm, p = 0.0004; 23.5 +/- 21.3 mug g(-1) creatinine versus 12.6 +/- 13.2 mug g(-1) creatinine, p = 0.028). (c) 2004 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | performance liquid-chromatography ; capillary-zone-electrophoresis ; column amperometric detection ; microdisk array electrode ; solid-phase extraction ; dynamic ph junction ; electrochemical detection ; gas-chromatography ; cancer-patients ; 8-hydroxydeoxyguanosine |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000221735500018 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/81591]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China 2.Wuhan Univ, Coll Chem, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yao, QH,Mei, SR,Weng, QF,et al. Determination of urinary oxidative DNA damage marker 8-hydroxy-2 '-deoxyguanosine and the association with cigarette smoking[J]. talanta,2004,63(3):617-623. |
| APA | Yao, QH.,Mei, SR.,Weng, QF.,Zhang, PD.,Yang, Q.,...&Xu, GW.(2004).Determination of urinary oxidative DNA damage marker 8-hydroxy-2 '-deoxyguanosine and the association with cigarette smoking.talanta,63(3),617-623. |
| MLA | Yao, QH,et al."Determination of urinary oxidative DNA damage marker 8-hydroxy-2 '-deoxyguanosine and the association with cigarette smoking".talanta 63.3(2004):617-623. |
入库方式： OAI收割
来源：大连化学物理研究所
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